使用寡核苷酸探针进行牛的DNA指纹分析。

DNA fingerprinting in cattle using oligonucleotide probes.

作者信息

Buitkamp J, Zischler H, Epplen J T, Geldermann H

机构信息

Institut für Tierzucht and Vererbungsforschung, Abteilung Haustiergenetik der Tierärztlichen Hochschule Hannover, Germany.

出版信息

Anim Genet. 1991;22(2):137-46. doi: 10.1111/j.1365-2052.1991.tb00655.x.

DOI:10.1111/j.1365-2052.1991.tb00655.x

PMID:1654029

Abstract

Oligonucleotide probes specific for simple tandem repeat sequences produce individual specific DNA fingerprints in man and all animal species tested so far. Here 11 different synthetic probes were hybridized to bovine genomic DNAs which had been digested with the restriction endonucleases HinfI, AluI and HaeIII. Two of these probes gave DNA fingerprint patterns which were analysed for three German breeds. Different parameters were calculated, such as the average number of bands per individual or the probability of finding identical fingerprints in two unrelated individuals. The number of polymorphic bands varies from 11 to 23 in the different breeds and the probability of finding the same banding pattern in two unrelated individuals ranges from 1.5 x 10(-7) to 2.4 x 10(-7). Hence this DNA fingerprinting procedure allows precise identification of individuals. It is also a useful additional method for paternity testing in cattle.

摘要

针对简单串联重复序列的寡核苷酸探针，在人类以及目前所有已测试的动物物种中，都能产生个体特异性的DNA指纹图谱。在此，将11种不同的合成探针与经限制性内切酶HinfI、AluI和HaeIII消化的牛基因组DNA进行杂交。其中两种探针产生了DNA指纹图谱，并对三个德国品种进行了分析。计算了不同参数，如每个个体的平均条带数，或在两个不相关个体中发现相同指纹的概率。不同品种中多态性条带的数量在11至23条之间变化，在两个不相关个体中发现相同条带模式的概率在1.5×10⁻⁷至2.4×10⁻⁷之间。因此，这种DNA指纹图谱技术能够精确鉴定个体。它也是牛亲子鉴定中一种有用的辅助方法。