Poincloux Renaud, Vincent Claire, Labrousse Arnaud, Castandet Jerôme, Rigo Maxime, Cougoule Céline, Bordier Christine, Le Cabec Véronique, Maridonneau-Parini Isabelle
CNRS UMR5089, Institut de Pharmacologie et de Biologie Structurale, 205 route de Narbonne, F-31077 Toulouse Cedex, France.
Eur J Cell Biol. 2006 Apr;85(3-4):327-32. doi: 10.1016/j.ejcb.2005.09.012. Epub 2005 Oct 19.
Podosomes are adhesion structures with an extracellular matrix-degrading capacity mostly found in monocyte-derived cells. We have previously shown that the protein tyrosine kinase Hck, a member of the Src family, triggers the de novo formation of podosome rosettes in a lysosome-dependent manner when expressed in its constitutively active form. Hck is specifically expressed in myeloid cells. In human monocyte-derived macrophages (MDMs) it is present at podosomes. Here we addressed whether its activation by lipopolysaccharide and interferon-gamma has an effect on podosome organization in MDMs. Several structures were observed evolving from individual podosomes to clusters, aggregates and rosettes. In chronic myeloid leukemia cells, Hck is constitutively activated by the fusion protein Bcr-Abl and podosome-like structures were present. Finally, in monocyte-derived osteoclasts, Hck was found to accumulate at podosome belts. In conclusion, in monocyte-derived cells, it is likely that Hck could play a role in podosome re-arrangements.
足体是一种具有细胞外基质降解能力的粘附结构，主要存在于单核细胞衍生的细胞中。我们之前已经表明，蛋白酪氨酸激酶Hck是Src家族的成员之一，当其以组成型活性形式表达时，会以溶酶体依赖的方式触发足体玫瑰花结的从头形成。Hck在髓系细胞中特异性表达。在人单核细胞衍生的巨噬细胞（MDM）中，它存在于足体中。在这里，我们探讨了脂多糖和干扰素-γ对其的激活是否会影响MDM中足体的组织。观察到几种结构从单个足体演变为簇、聚集体和玫瑰花结。在慢性髓性白血病细胞中，Hck被融合蛋白Bcr-Abl组成型激活，并且存在足体样结构。最后，在单核细胞衍生的破骨细胞中，发现Hck在足体带处积累。总之，在单核细胞衍生的细胞中，Hck很可能在足体重排中发挥作用。
