Departments of Microbiology and Veterinary Pathology, University of Illinois, Urbana, Illinois 61801.
Infect Immun. 1974 Nov;10(5):1127-34. doi: 10.1128/iai.10.5.1127-1134.1974.
Organ cultures of adult hamster trachea were used to evaluate the cytotoxic potential of cell fractions of Mycoplasma pneumoniae. Cytoplasm was essentially devoid of activity, whereas viable cells and membrane preparations, at a level of 25 mug of protein per ml, induced necrosis. Damage, as revealed by light and electron microscopy, included ciliostasis, vacuolization, loss of ciliated respiratory epithelial cells, disorganization, and a loss of polarity. Dose response data indicated that the speed and degree of cytotoxicity was directly related to the concentration of membranes. Doses of 30 to 60 mug of protein per ml could reduce relative ciliary activity to 20% of the control level within 4 days. Membranes prepared after freeze-thaw lysis of cells were almost twice as active as those isolated after a combination of osmotic and sonic shock. Membranes of M. fermentans were inactive, though both the FH and M129 strains of M. pneumoniae were toxic. These data indicate that the toxic factor responsible for M. pneumoniae may be located in the cell membrane.
采用成年仓鼠气管器官培养物来评估肺炎支原体细胞成分的细胞毒性潜能。细胞质基本没有活性，而活细胞和膜制剂在每毫升 25 微克蛋白的水平上，诱导了坏死。光镜和电镜下的损伤包括纤毛停滞、空泡化、纤毛呼吸上皮细胞丧失、结构紊乱和极性丧失。剂量反应数据表明，细胞毒性的速度和程度与膜的浓度直接相关。在 4 天内，每毫升 30 至 60 微克蛋白的剂量可将相对纤毛活性降低至对照水平的 20%。用细胞冻融裂解制备的膜比用渗透压和超声冲击联合分离的膜活性高近两倍。M. fermentans 的膜没有活性，尽管肺炎支原体的 FH 和 M129 株均有毒。这些数据表明，导致肺炎支原体毒性的因素可能位于细胞膜上。
