Comparative study between the Light Cycler and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations.
作者信息
Mammo Layla, Sheereen Atia, Saour Tanya, Saour Jalal
机构信息
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia.
出版信息
Clin Biochem. 2006 Jul;39(7):767-9. doi: 10.1016/j.clinbiochem.2006.01.019. Epub 2006 Mar 23.
Abstract
OBJECTIVES
To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice.
DESIGN AND METHODS
We compared conformity, reproducibility, speed and cost using the Light Cycler (LC) and PCR-RFLP.
RESULTS
There was 100% conformity and reproducibility. LC was faster but 23% more expensive per sample. When equipment depreciation and patient expenses are added, LC testing becomes cheaper.
CONCLUSION
In our practice, LC provides fast, reproducible and cost-effective results.
摘要
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