β1,4-半乳糖基转移酶V表达的下调促进整合素β1成熟。
Down-regulation of the expression of beta1,4-galactosyltransferase V promotes integrin beta1 maturation.
作者信息
Chen Xiaoning, Jiang Jianhai, Yang Junwu, Chen Chun, Sun Maoyun, Wei Yuanyan, Guang Xiaoying, Gu Jianxin
机构信息
Key Laboratory of Medical Molecular Virology Ministry of Education and Health, Gene Research Center, Shanghai Medical College and Institutes of Biomedical Sciences of Fudan University, Shanghai 200032, PR China.
出版信息
Biochem Biophys Res Commun. 2006 May 12;343(3):910-6. doi: 10.1016/j.bbrc.2006.03.046. Epub 2006 Mar 20.
In previous study, we have shown that beta1,4-galactosyltransferase V (GalT V) functions as a positive growth regulator in glioma. Here, we reported that down-regulation of the expression of GalT V in SHG44 cells by transfection with antisense cDNA specifically up-regulated the expression of cell surface integrin beta1 without the change of its mRNA, and with integrin beta1 125 kDa mature form increased and 105 kDa precursor form decreased. It is well known that the N-glycans of integrins modulate the location and functions of integrins. The SHG44 cells transfected with antisense cDNA of GalT V demonstrated decreased Golgi localization of integrin beta1, strengthened the interaction between integrin alpha5 and beta1 subunit, and enhanced the adhesion ability to fibronectin and the level of focal adhesion kinase phosphorylation. Our results suggested that the down-regulation of the expression of GalT V could promote the expression of cell surface integrin beta1 and subsequently inhibit glioma malignant phenotype.
在先前的研究中，我们已经表明β1,4-半乳糖基转移酶V（GalT V）在胶质瘤中作为一种正向生长调节因子发挥作用。在此，我们报告通过用反义cDNA转染来下调SHG44细胞中GalT V的表达，可特异性地上调细胞表面整合素β1的表达，而其mRNA无变化，且整合素β1的125 kDa成熟形式增加，105 kDa前体形式减少。众所周知，整合素的N-聚糖可调节整合素的定位和功能。用GalT V反义cDNA转染的SHG44细胞显示整合素β1的高尔基体定位减少，增强了整合素α5与β1亚基之间的相互作用，并增强了对纤连蛋白的黏附能力以及粘着斑激酶磷酸化水平。我们的结果表明，GalT V表达的下调可促进细胞表面整合素β1的表达，进而抑制胶质瘤的恶性表型。
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