Clifton Matthew S, Joe Bonnie N, Zektzer Andrew S, Kurhanewicz John, Vigneron Daniel B, Coakley Fergus V, Nobuhara Kerilyn K, Swanson Mark G
Department of Surgery, University of California-San Francisco, San Francisco, CA 94143-0570, USA.
J Pediatr Surg. 2006 Apr;41(4):768-73. doi: 10.1016/j.jpedsurg.2006.02.020.
BACKGROUND/PURPOSE: Amniocentesis is an invasive procedure with inherent risks. Magnetic resonance (MR) spectroscopy is a safe noninvasive way of measuring levels of choline-containing compounds (including surfactant) and other metabolites. The purpose of this study was to test the feasibility of assessing fetal lung maturity in vivo and ex vivo using MR spectroscopy to determine differences in amniotic fluid choline concentrations between the second and third trimesters.
Magnetic resonance spectroscopy was performed on ex vivo samples of amniotic fluid from second- and third-trimester fetuses. In vivo MR spectroscopy was performed on amniotic fluid and fetal lungs in third-trimester fetuses. Spectral acquisition and analysis were performed by an attending radiologist in conjunction with an MR spectroscopist.
Choline-containing compounds were observed from 3.20 to 3.25 ppm. Comparison of spectra from second- and third-trimester amniocentesis revealed a trend toward increased choline at later gestational ages. Spectra from amniotic fluid and lungs of a third-trimester fetus showed that choline can be detected in the in vivo setting.
Magnetic resonance spectroscopy is a safe noninvasive procedure that enables measurement of choline-containing compounds in fetal lung and amniotic fluid. Magnetic resonance spectroscopy shows a trend toward an increased quantity of choline in third- vs second-trimester amniocentesis.
背景/目的：羊膜腔穿刺术是一种具有固有风险的侵入性操作。磁共振（MR）波谱分析是一种安全的非侵入性方法，可用于测量含胆碱化合物（包括表面活性剂）和其他代谢物的水平。本研究的目的是测试使用MR波谱分析在体内和体外评估胎儿肺成熟度的可行性，以确定孕中期和孕晚期羊水胆碱浓度的差异。
对孕中期和孕晚期胎儿的羊水离体样本进行磁共振波谱分析。对孕晚期胎儿的羊水和胎儿肺进行体内磁共振波谱分析。光谱采集和分析由一位主治放射科医生与一位MR波谱分析专家共同进行。
在3.20至3.25 ppm处观察到含胆碱化合物。孕中期和孕晚期羊膜腔穿刺术光谱的比较显示，在妊娠后期胆碱有增加的趋势。孕晚期胎儿羊水和肺的光谱显示，在体内环境中可以检测到胆碱。
磁共振波谱分析是一种安全的非侵入性操作，能够测量胎儿肺和羊水中的含胆碱化合物。磁共振波谱分析显示，与孕中期羊膜腔穿刺术相比，孕晚期羊水中胆碱含量有增加的趋势。
