使用Streamline Direct CST I吸附剂对蛋白质进行扩张床吸附/解吸

Expanded bed adsorption/desorption of proteins with Streamline Direct CST I adsorbent.

作者信息

Li Ping, Xiu Guohua, Mata Vera G, Grande Carlos A, Rodrigues Alirio E

机构信息

Department of Chemical Engineering, Laboratory of Separation and Reaction Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n 4200-465 Porto, Portugal.

出版信息

Biotechnol Bioeng. 2006 Aug 20;94(6):1155-63. doi: 10.1002/bit.20952.

DOI:10.1002/bit.20952

PMID:16572450

Abstract

Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out for two-component proteins (BSA and myoglobin) competitive adsorption and desorption in an expanded bed packed with Streamline Direct CST I. Based on the measurements of the single- and two-component bovine serum albumin (BSA)/myoglobin adsorption isotherm on Streamline Direct CST I, the binding and elution conditions for the whole EBA process are selected; and then frontal analysis for a longer timescale and column displacement experiments in a fixed bed (XK16/20 column) are carried out to evaluate the two-component proteins (BSA and myoglobin) competitive adsorption and displacement on Streamline Direct CST I. Finally, the feasibility of capturing both BSA and myoglobin by an expanded bed packed with Streamline Direct CST I is addressed in a Streamline 50 column packed with 300 mL Streamline Direct CST I.

摘要

Streamline Direct CST I是一种新型的具有多模态官能团的离子交换剂，专为膨胀床吸附（EBA）工艺设计，能够以高结合容量直接从高离子强度原料中捕获蛋白质。在本研究中，对在填充有Streamline Direct CST I的膨胀床中两组分蛋白质（牛血清白蛋白和肌红蛋白）的竞争吸附和解吸进行了实验研究。基于在Streamline Direct CST I上对单组分和两组分牛血清白蛋白（BSA）/肌红蛋白吸附等温线的测量，选择了整个EBA工艺的结合和洗脱条件；然后进行了更长时间尺度的前沿分析以及在固定床（XK16/20柱）中的柱置换实验，以评估两组分蛋白质（BSA和肌红蛋白）在Streamline Direct CST I上的竞争吸附和置换。最后，在填充有300 mL Streamline Direct CST I的Streamline 50柱中探讨了用填充有Streamline Direct CST I的膨胀床捕获BSA和肌红蛋白的可行性。