Itakura Shuji, Masuta Takafumi, Tanaka Hiromi, Enoki Akio
Department of Applied Biological Chemistry, Kinki University, Naka-Machi, Nara, 631-8505, Japan.
J Econ Entomol. 2006 Feb;99(1):123-8. doi: 10.1093/jee/99.1.123.
To identify subterranean termite species, we designed a pair of common primers that amplified 381-bp fragments from cDNAs encoding the endo-beta-1,4-glucanases (EGases) of Coptotermes formosanus Shiraki and Reticulitermes speratus (Kolbe). cDNAs from C. formosanus and R. speratus, and genomic DNA from R. speratus, were amplified by polymerase chain reaction (PCR) by using this primer pair and then cloned and sequenced. Sequences amplified from C.formosanus cDNA displayed 97-99% identity to cDNA encoding the EGase of C. formosanus (CfEG), and 92-94% identity to cDNA encoding the EGase of R. speratus (RsEG). By contrast, cDNA from R. speratus displayed 99-100% identity to RsEG cDNA and 93-94% identity to CfEG cDNA. CfEG and RsEG cDNAs can therefore be used as markers for the identification of these termite species. This is the first report of the successful identification of termite species by using cDNA and genomic DNA sequences of termite origin.
为了鉴定地下白蚁种类，我们设计了一对通用引物，该引物能从编码台湾乳白蚁（Coptotermes formosanus Shiraki）和黑胸散白蚁（Reticulitermes speratus (Kolbe)）内切β-1,4-葡聚糖酶（EGases）的cDNA中扩增出381 bp的片段。使用该引物对通过聚合酶链反应（PCR）扩增台湾乳白蚁和黑胸散白蚁的cDNA以及黑胸散白蚁的基因组DNA，然后进行克隆和测序。从台湾乳白蚁cDNA扩增出的序列与编码台湾乳白蚁EGase（CfEG）的cDNA具有97 - 99%的同一性，与编码黑胸散白蚁EGase（RsEG）的cDNA具有92 - 94%的同一性。相比之下，黑胸散白蚁的cDNA与RsEG cDNA具有99 - 100%的同一性，与CfEG cDNA具有93 - 94%的同一性。因此，CfEG和RsEG cDNA可作为这些白蚁种类鉴定的标记。这是首次利用白蚁来源的cDNA和基因组DNA序列成功鉴定白蚁种类的报告。
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