Department of Chemistry and Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720.
Proc Natl Acad Sci U S A. 1983 Feb;80(3):683-6. doi: 10.1073/pnas.80.3.683.
Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. This procedure allows for an initial recovery of 80-90% of the bacterial reaction centers present in chromatophore membranes. High purity reaction centers (A(280)/A(802) < 1.30) can be obtained with a 30% recovery. Reaction centers from wild-type Rps. sphaeroides and Rps. capsulata also bind to a cytochrome c column. Cytochrome c affinity chromatography can also be used to isolate photosystem I complexes from spinach chloroplasts.
马心细胞色素 c 与 Sepharose 4B 相连，用于从球形红假单胞菌 R-26 中纯化反应中心。该程序允许从类囊体膜中存在的细菌反应中心初始回收 80-90%。通过 30%的回收率可以获得高纯度的反应中心（A(280)/A(802) < 1.30）。野生型 Rps. sphaeroides 和 Rps. capsulata 的反应中心也与细胞色素 c 柱结合。细胞色素 c 亲和层析也可用于从菠菜叶绿体中分离光系统 I 复合物。
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