Department of Biochemistry, University of Missouri, Columbia, MO 65211.
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9128-30. doi: 10.1073/pnas.84.24.9128.
An apparently defective bacteriophage capable of mediating transduction has been identified in culture filtrates of Desulfovibrio desulfuricans (American Type Culture Collection 27774). Phage-mediated intraspecies transfer of antibiotic resistance markers occurs with a frequency of 10(-5) to 10(-6) per recipient cell. The vector contains linear fragments of double-stranded DNA of about 13.5 kilobase pairs, which appear to be random pieces of bacterial DNA. As yet, neither induction nor plaque formation has been observed. To our knowledge, a system of genetic exchange has not been described before for a member of the sulfate-reducing bacteria.
在脱硫弧菌(美国模式培养物集存库 27774)的培养液滤出物中,发现了一种显然有缺陷但能介导转导的噬菌体。噬菌体介导的同种抗生素抗性标记的转移,在每一个受体细胞中发生的频率为 10(-5)到 10(-6)。载体含有约 13500 对碱基的双链 DNA 的线性片段,这些片段似乎是细菌 DNA 的随机片段。到目前为止,还没有观察到诱导或噬菌斑的形成。据我们所知,对于硫酸盐还原菌来说,这种遗传交换系统以前还没有被描述过。