Hinson J A, Neal R A
Biochim Biophys Acta. 1975 Mar 28;384(1):1-11. doi: 10.1016/0005-2744(75)90090-x.
The kinetics of the horse liver alcohol dehydrogenase (alcohol: NAD+ oxidoreductase EC 1.1.1.1) catalyzed metabolism of octanol and octanal to octanoic acid have been examined. On incubation of octanol with horse liver alcohol dehydrogenase in the presence of NAD+, NADH as well as octanal and octanoic acid were seen as the initial products. However, on continued incubation, the octanal concentration progressively decreased to where only negligible quantities were present in the incubation after 10 min. The production of NADH was biphasic. An initial phase was followed in about 2 min with a slower but linear rate of NADH production. The production of octanoic acid was approximately linear throughout the 10 min incubation period. Since octanal is an intermediate in the oxidation of octanol to octanoic acid, the ability of semicarbazide to inhibit the metabolism of octanol to octanoic acid was examined. At a concentration of semicarbazide which was 63 times the concentration of octanol in the incubation media, the rate of formation of octanoic acid was inhibited by only 30%. The results of these experiments suggest that in the oxidation of octanol to octanoic acid a portion of the octanal formed from octanol is not released from the enzyme but, in the presence of NAD+, is oxidized to octanoic acid.
已对马肝醇脱氢酶（乙醇：NAD⁺氧化还原酶，EC 1.1.1.1）催化辛醇和辛醛代谢为辛酸的动力学进行了研究。在NAD⁺存在的情况下，将辛醇与马肝醇脱氢酶一起孵育时，NADH以及辛醛和辛酸被视为初始产物。然而，持续孵育后，辛醛浓度逐渐降低，10分钟后孵育体系中仅存在少量辛醛。NADH的产生呈双相性。最初约2分钟内为一个阶段，随后NADH的产生速率较慢但呈线性。在整个10分钟的孵育期内，辛酸的产生大致呈线性。由于辛醛是辛醇氧化为辛酸过程中的中间产物，因此研究了氨基脲抑制辛醇代谢为辛酸的能力。在孵育介质中，氨基脲的浓度是辛醇浓度的63倍时，辛酸的形成速率仅被抑制了30%。这些实验结果表明，在辛醇氧化为辛酸的过程中，由辛醇形成的一部分辛醛并未从酶中释放出来，而是在NAD⁺存在的情况下被氧化为辛酸。
