Longo G P, Scandalios J G
ATOMIC ENERGY COMMISSION PLANT RESEARCH LABORATORY, MICHIGAN STATE UNIVERSITY, EAST LANSING.
Proc Natl Acad Sci U S A. 1969 Jan;62(1):104-11. doi: 10.1073/pnas.62.1.104.
The isozymes of malic dehydrogenase (MDH) of maize have been separated by starch gel zone electrophoresis. The MDH isozyme pattern was the same in different organs and developmental stages. The subcellular distribution of the different isozymes was established by means of differential centrifugation. The MDH isozymes were found to be differentially distributed in the cytoplasm, the mitochondria, and the glyoxysomes. The latter proved the only exception to the pattern constancy among organs insofar as they are present only in the scutella. Among several inbred maize strains examined, only variants of the mitochondrial isozymes were found. Crosses between strains with different isozyme patterns gave Mendelian inheritance: thus, these mitochondrial isozymes are under nuclear-gene control.
通过淀粉凝胶区带电泳分离了玉米苹果酸脱氢酶（MDH）的同工酶。不同器官和发育阶段的MDH同工酶谱相同。通过差速离心确定了不同同工酶的亚细胞分布。发现MDH同工酶在细胞质、线粒体和乙醛酸循环体中呈差异分布。就其仅存在于盾片中而言，后者是器官间模式恒定的唯一例外。在所检测的几个玉米自交系中，仅发现了线粒体同工酶的变体。具有不同同工酶模式的品系间杂交呈现孟德尔遗传：因此，这些线粒体同工酶受核基因控制。
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