Weinberg E S, Voeller B R
THE ROCKEFELLER UNIVERSITY, NEW YORK, NEW YORK.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):835-42. doi: 10.1073/pnas.64.3.835.
Light-induced germination of Anemia spores can be inhibited by AMO-1618, a selective inhibitor of gibberellin biosynthesis. The inhibitor has no effect on gibberellin-induced dark germination and its inhibition of light-induced germination can be reversed by supplying gibberellin. Barley-endosperm bioassay of concentrates of medium in which spores are imbibed in light reveals the presence of substances with gibberellin-like activity; assay of medium from dark-imbibed spores does not. Simultaneous exposure of spores to sub-optimal levels of light and gibberellin leads to additivity of effect on germination level. Uptake of labeled gibberellin by spores in light is similar to that in darkness. The implications of these findings for the light-dependent synthesis of a gibberellin-like germination substance are discussed. The bearing of the observations upon understanding the interaction of light and gibberellins in seeds of higher plants is considered.
赤霉素生物合成的选择性抑制剂AMO - 1618能够抑制光诱导的贫血孢子萌发。该抑制剂对赤霉素诱导的暗萌发没有影响，并且通过供应赤霉素可以逆转其对光诱导萌发的抑制作用。对在光照下浸泡孢子的培养基浓缩物进行大麦胚乳生物测定，结果显示存在具有赤霉素样活性的物质；而对在黑暗中浸泡孢子的培养基进行测定则未发现此类物质。同时将孢子暴露于次最佳水平的光照和赤霉素下，对萌发水平产生累加效应。光照下孢子对标记赤霉素的吸收与黑暗中相似。本文讨论了这些发现对于光依赖性合成赤霉素样萌发物质的意义。还考虑了这些观察结果对于理解高等植物种子中光与赤霉素相互作用的影响。
Zotero 插件