发芽棉花胚根中核DNA复制的机制

Mechanism of nuclear DNA replication in radicles of germinating cotton.

作者信息

Clay W F, Bartels P G, Katterman F R

机构信息

Department of Plant Sciences, University of Arizona, Tucson, Ariz. 85721.

出版信息

Proc Natl Acad Sci U S A. 1976 Sep;73(9):3220-3. doi: 10.1073/pnas.73.9.3220.

DOI:10.1073/pnas.73.9.3220

PMID:16592350

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430984/

Abstract

The mechanism of nuclear DNA replication in radicles of germinating cotton (Gossypium barbadense) was investigated. Pulse-labeling with [(3)H]thymidine indicates that replication intermediates are of small molecular weight (4-10S) and behave as if single-stranded. Prolonged labeling indicates that intermediates are of discrete size, suggesting a mechanism of discontinuous replication. Electron microscopy of nuclear DNA demonstrates a complex architecture which may include "eye forms" and "forks" similar to those reported in prokaryotes and animal systems. The possible universality of DNA replication mechanisms is discussed.

摘要

对发芽棉花（海岛棉）胚根中核DNA复制机制进行了研究。用[³H]胸腺嘧啶核苷进行脉冲标记表明，复制中间体分子量较小（4 - 10S），且表现得好像是单链的。长时间标记表明中间体具有离散大小，提示存在不连续复制机制。核DNA的电子显微镜观察显示出一种复杂的结构，其中可能包括类似于原核生物和动物系统中报道的“眼状结构”和“叉状结构”。文中讨论了DNA复制机制可能的普遍性。