Dickson E, Diener T O, Robertson H D
The Rockefeller University, New York, New York 10021.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):951-4. doi: 10.1073/pnas.75.2.951.
Potato spindle tuber viroid and citrus exocortis viroid, each purified from tomato (Lycopersicon esculentum) and from Gynura aurantiaca, were iodinated in vitro with (125)I, digested with ribonuclease T1, and subjected to two-dimensional RNA fingerprinting analysis. With the exception of minor variations, each viroid retained its distinctive fingerprint pattern irrespective of the host species from which it was isolated. We conclude that the nucleotide sequences of these viroids are principally determined by the infecting viroid and not by the host.
从番茄(番茄)和紫背天葵中分别纯化得到的马铃薯纺锤块茎类病毒和柑橘裂皮类病毒,在体外经碘-125碘化,用核糖核酸酶T1消化,然后进行二维RNA指纹图谱分析。除了一些细微差异外,每种类病毒都保留了其独特的指纹图谱模式,无论它是从哪种宿主物种中分离出来的。我们得出结论,这些类病毒的核苷酸序列主要由感染的类病毒决定,而非宿主。