Ghisla S, Hastings J W, Favaudon V, Lhoste J M
Fachbereich Biologie, Universität Konstanz, D-775 Konstanz, Germany.
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5860-3. doi: 10.1073/pnas.75.12.5860.
By using FMN enriched in (13)C (90%) at position C-4a, we have conclusively shown that the reaction of molecular oxygen with bacterial luciferase-bound FMNH(2) forms an adduct at the 4a position. Consistent with this are (13)C NMR studies of FMN and other flavin compounds which show that this carbon should be unusually reactive in the reduced 1,5-dihydroflavins with respect to electrophilic attacks.
通过使用在C-4a位富含(90%)(13)C的FMN,我们已经确凿地表明,分子氧与细菌荧光素酶结合的FMNH₂的反应在4a位形成了加合物。与此一致的是,对FMN和其他黄素化合物的(13)C NMR研究表明,在还原型1,5 - 二氢黄素中,该碳原子对于亲电攻击应该具有异常的反应活性。