Whitmarsh J, Cramer W A
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4417-20. doi: 10.1073/pnas.76.9.4417.
The high-potential iron-sulfur center associated with the g = 1.89-1.90 electron paramagnetic resonance signal in spinach chloroplasts is fully reduced in the dark. It is photooxidized in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea and reduced after illumination with red actinic light in the absence of inhibitors. The light-induced redox changes of the high-potential iron-sulfur center are shown to correlate with those of plastocyanin detected at g = 2.05. Demonstration of light-induced redox changes in the high-potential iron-sulfur center provides support for a functional role of this component in the chloroplast electron transport chain.
与菠菜叶绿体中g = 1.89 - 1.90电子顺磁共振信号相关的高电位铁硫中心在黑暗中完全还原。在3-(3,4-二氯苯基)-1,1-二甲基脲存在下它被光氧化,并且在没有抑制剂的情况下用红光照射后还原。高电位铁硫中心的光诱导氧化还原变化与在g = 2.05处检测到的质体蓝素的氧化还原变化相关。高电位铁硫中心光诱导氧化还原变化的证明为该组分在叶绿体电子传递链中的功能作用提供了支持。