Department of Biology, University of Calgary, Calgary T2N 1N4, Alberta, Canada.
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3408-11. doi: 10.1073/pnas.77.6.3408.
cis-4-Cyclohexene-1,2-dicarboximide (CHDC) inhibits the germination of light-requiring seeds in both light and darkness but has no effect upon the germination of non-light-requiring seeds. In lettuce seeds, CHDC inhibits the action of far-red-absorbing form of phytochrome in breaking dormancy. This inhibition can be overcome by benzyladenine and red light together, but not by a combination of red light and gibberellic acid. Gibberellic acid-induced germination of lettuce seeds in darkness is inhibited also by CHDC. Embryos isolated from dark-imbibed lettuce seeds germinate on the inhibitor. CHDC was thought to be an "analogue" of cycloheximide, but it does not inhibit protein synthesis in lettuce seeds. Our results lead us to conclude that CHDC inhibits germination of seeds that require red light to break dormancy and interferes with some aspect of metabolism that is stimulated by far-red-absorbing form of phytochrome.
顺-4-环己烯-1,2-二羧酸亚胺（CHDC）抑制需光种子在光照和黑暗中的萌发，但对非需光种子的萌发没有影响。在莴苣种子中，CHDC 抑制远红光吸收型光敏色素打破休眠的作用。这种抑制可以被苄基腺嘌呤和红光共同克服，但不能被红光和赤霉素的组合克服。赤霉素诱导的黑暗中莴苣种子的萌发也被 CHDC 抑制。从黑暗吸胀的莴苣种子中分离出的胚在抑制剂上萌发。CHDC 被认为是环己亚胺的“类似物”，但它不抑制莴苣种子中的蛋白质合成。我们的结果表明，CHDC 抑制需要红光来打破休眠的种子的萌发，并干扰远红光吸收型光敏色素刺激的代谢的某个方面。
