Institute of Biological Chemistry and Program in Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3536-40. doi: 10.1073/pnas.78.6.3536.
The synthesis and accumulation of proteinase inhibitor I in excised tomato leaves can be induced with oligosaccharides obtained by fungal endo-alpha-1,4-polygalacturonase digestion of a pectic polysaccharide (M(r) 5000-10,000) isolated from tomato leaves. Active oligosaccharides were also released from isolated tomato leaf cell walls by endopolygalacturonases partially purified from tomato plants. It is suggested that oligosaccharides, released from plant cell wall pectic polysaccharides by either endogenous or exogenous endopolygalacturonases at a wound or infection site, may have hormone-like roles in regulating plant defense responses in unwounded tissues many centimeters away from the site of release.
从番茄叶片中分离得到的相对分子质量为 5000-10000 的果胶多糖,用真菌内切-α-1,4-多聚半乳糖醛酸酶消化后可得到寡糖,这种寡糖可诱导番茄叶片中蛋白酶抑制剂 I 的合成和积累。从番茄植株中部分纯化的内切多聚半乳糖醛酸酶也可从分离的番茄叶细胞壁中释放出有活性的寡糖。因此,在伤口或感染部位,无论是内源的还是外源的内切多聚半乳糖醛酸酶从植物细胞壁的果胶多糖中释放出的寡糖,可能在调节远离释放部位的数厘米外未受伤组织的植物防御反应方面具有激素样作用。