Department of Entomology, University of Florida, Gainesville, Florida 32611.
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2589-93. doi: 10.1073/pnas.79.8.2589.
A cell line has been developed from the imaginal wing discs of the lepidopteran insect Trichoplusia ni (Hübner). The cells grow in suspension as fluid-filled, multicellular vesicles. The cell line has been designated IAL-TND1. Two-dimensional electrophoresis (isoelectric focusing and sodium dodecyl sulfate/polyacrylamide gels) of [(35)S]methionine-labeled proteins revealed similar patterns for IAL-TND1 cells and whole T. ni wing imaginal discs. Additionally, exposure to the insect steroid hormone 20-hydroxyecdysone stimulated the synthesis of the same new proteins in both whole wing discs and IAL-TND1 cell vesicles.
已从小翅目昆虫烟夜蛾（Trichoplusia ni （Hübner））的成虫翅盘上开发出一种细胞系。这些细胞作为充满液体的多细胞囊泡在悬浮液中生长。该细胞系被命名为 IAL-TND1。[(35)S]甲硫氨酸标记蛋白的二维电泳（等电聚焦和十二烷基硫酸钠/聚丙烯酰胺凝胶）显示 IAL-TND1 细胞和整个烟夜蛾成虫翅盘的蛋白质图谱相似。此外，暴露于昆虫甾体激素 20-羟基蜕皮酮可刺激整个翅盘和 IAL-TND1 细胞囊泡中相同新蛋白质的合成。
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