Imperial Cancer Research Fund, Mill Hill Laboratories, Burtonhole Lane, London NW7 1AD, United Kingdom.
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5350-4. doi: 10.1073/pnas.80.17.5350.
Restriction maps for 25 kilobases of DNA around the 87A7 heat shock locus have been determined in 29 chromosomes isolated from a natural population. The heterozygosity per nucleotide and the proportion of polymorphic nucleotide sites were estimated to be 0.0024 and 0.007, respectively. The mean number of insertional differences in this region between random pairs of chromosomes was 0.95. A significant amount of this variation was due to the insertion of large transposable elements. All the insertion/deletion events were found in a region less than 2 kilobases in size. This could either be due to nonrandom integration or to differences in the intensity of selection against DNA insertion at different sites.
已在从自然种群中分离出的 29 条染色体上确定了围绕 87A7 热休克基因座的 25 千碱基 DNA 的限制图谱。每个核苷酸的杂合度和多态核苷酸位点的比例估计分别为 0.0024 和 0.007。在该区域,随机两条染色体之间的插入差异平均数为 0.95。这种变异的很大一部分是由于大转座因子的插入。所有的插入/缺失事件都发生在不到 2 千碱基大小的区域内。这可能是由于非随机整合,也可能是由于不同部位 DNA 插入的选择强度不同。