Department of Plant Genetics. The Weizmann Institute of Science, Rehovot 76100, Israel.
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1485-9. doi: 10.1073/pnas.82.5.1485.
A high rate of plastome-encoded mutations was induced in Nicotiana by exposing seeds to N-nitroso-N-methylurea. Seeds then were subjected to nutritional and in vitro selection procedures for systematic isolation of plastome-dependent antibiotic-resistant plants. Multiple flowering lines resistant to streptomycin, spectinomycin, lincomycin, or chloramphenicol were obtained. A detailed analysis of the streptomycin-resistant lines is presented. Sexual hybridization, cybrid formation following protoplast fusion, and in organello protein synthesis were used to rigorously assign the mutations to the chloroplast genome. The efficient rates of mutagenesis combined with the in vitro mass-screening procedures described here should facilitate investigation of fundamental aspects of chloroplast genetics in higher plants.
用 N-亚硝基-N-甲基脲处理烟草种子可诱导大量质体编码突变。然后对种子进行营养和体外选择程序，以系统分离质体依赖性抗生素抗性植物。获得了对链霉素、壮观霉素、林可霉素或氯霉素具有抗性的多种开花系。本文介绍了对链霉素抗性系的详细分析。利用有性杂交、原生质体融合后的胞质杂种形成以及细胞器蛋白合成，将突变严格分配到叶绿体基因组上。这里描述的高效诱变率与体外大规模筛选程序相结合，应该有助于研究高等植物中叶绿体遗传学的基本方面。
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