Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019.
Proc Natl Acad Sci U S A. 1985 Sep;82(18):6129-32. doi: 10.1073/pnas.82.18.6129.
Gametic fusion patterns in the angiosperm Plumbago zeylanica were determined by using cytoplasmically dimorphic sperm cells differing in mitochondrion and plastid content and then identifying paternal organelles through their ultrastructural characteristics within the maternal cytoplasm at the time of fertilization. The virtual absence of plastids within the sperm cell that is physically associated with the vegetative nucleus allows paternal plastids to be used to trace the fate of the two male gametes after fusion. Such paternal plastids were present in the egg in >94% of the observed cases, indicating the preferential fusion of the plastid-rich, mitochondrion-poor sperm cell with the egg. In only one instance did the opposite pattern occur. Since the possibility of this result occurring as the consequence of chance in random fusions is <1 in 7000, this represents strong evidence for the presence of a final putative recognition event occurring at the gametic level.
通过使用细胞质二态性精子细胞来确定被子植物白花丹中的配子融合模式，这些精子细胞在线粒体和质体含量上存在差异，然后通过在受精时母细胞质内的超微结构特征来识别父系细胞器。与营养核物理相关的精子细胞内几乎不存在质体，这使得父系质体能够用于追踪融合后两个雄配子的命运。在观察到的情况下，超过 94%的情况下卵子中存在这种父系质体，表明富含质体、线粒体较少的精子细胞与卵子优先融合。只有一种情况出现了相反的模式。由于这种结果作为随机融合的偶然结果发生的可能性小于 1/7000，这代表了在配子水平上发生最终假定识别事件的有力证据。
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