分离含有 D-1 和 D-2 多肽以及细胞色素 b-559 的光系统 II 反应中心。

Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559.

机构信息

Department of Biology, Faculty of Science, Okayama University, Okayama 700, Japan.

出版信息

Proc Natl Acad Sci U S A. 1987 Jan;84(1):109-12. doi: 10.1073/pnas.84.1.109.

DOI:10.1073/pnas.84.1.109

PMID:16593792

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC304151/

Abstract

A photosystem II reaction center complex consisting of D-1 and D-2 polypeptides and cytochrome b-559 was isolated from spinach grana thylakoids, treated with 4% (wt/vol) Triton X-100, by ion-exchange chromatography using DEAE-Toyopearl 650S. The isolated complex appears to contain five chlorophyll a, two pheophytin a, one beta-carotene, and one or two cytochrome b-559 heme(s) (molar ratio) and exhibits a reversible absorbance change attributable to the photochemical accumulation of reduced pheophytin typical for the intermediary electron acceptor of photosystem II reaction center. These results strongly suggest that the site of primary charge separation in photosystem II is located on the heterodimer composed of D-1 and D-2 subunits.

摘要

从菠菜类囊体基质中分离出一种由 D-1 和 D-2 多肽和细胞色素 b-559 组成的光系统 II 反应中心复合物，用 4%（重量/体积）Triton X-100 处理，通过离子交换层析用 DEAE-Toyopearl 650S 进行。分离出的复合物似乎含有五个叶绿素 a、两个脱镁叶绿素 a、一个β-胡萝卜素和一个或两个细胞色素 b-559 血红素（摩尔比），并表现出可归因于光化学积累的可逆吸收变化典型的光系统 II 反应中心中间电子受体的还原脱镁叶绿素。这些结果强烈表明，光系统 II 中最初电荷分离的位置位于 D-1 和 D-2 亚基组成的异二聚体上。