Salinardi B J, Roush J K, Schermerhorn T, Mitchell K E
Department of Clinical Science, Kansas State University, Manhattan 66506, USA.
Vet Comp Orthop Traumatol. 2006;19(1):49-55.
To better understand the mechanisms responsible for the pathological processes of osteoarthritis (OA) and to potentially identify a profile of changes that could be predictive of early OA, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in the synovial fluid and serum of normal and osteoarthritic dogs were examined. The concentration of MMP-1 in the synovial fluid of osteoarthritic dogs (0.62 +/- 0.16), as measured by densitometry, was significantly higher than that found in control dogs (0.42 +/- 0.19) (P = 0.03). The concentration of MMP-1 in the serum of osteoarthritic dogs (0.74 +/- 0.16) was significantly less than that found in control dogs (0.87 +/- 0.08) (P = 0.05). The concentration of TIMP-2 in the synovial fluid of osteoarthritic dogs (46.2 +/- 21.9 ng/ml) was significantly less than that of control dogs (122.0 +/- 66.5 ng/ml) (P = 0.009). The concentration of TIMP-2 in the serum of osteoarthritic dogs (116.2 +/- 43.1 ng/ml) was not significantly different than that of control dogs (95.1 +/- 94.4 ng/ml) (P = 0.554). In addition, a phospho-tyrosine immunoprecipitation and mass spectrometry were used to isolate and identify interferon-alpha in canine synovial fluid.
为了更好地理解骨关节炎（OA）病理过程的相关机制，并有可能确定可预测早期OA的变化特征，对正常犬和患骨关节炎犬的滑液及血清中的基质金属蛋白酶-1（MMP-1）和基质金属蛋白酶组织抑制剂-2（TIMP-2）进行了检测。通过光密度测定法测得，患骨关节炎犬滑液中MMP-1的浓度（0.62±0.16）显著高于对照犬（0.42±0.19）（P = 0.03）。患骨关节炎犬血清中MMP-1的浓度（0.74±0.16）显著低于对照犬（0.87±0.08）（P = 0.05）。患骨关节炎犬滑液中TIMP-2的浓度（46.2±21.9 ng/ml）显著低于对照犬（122.0±66.5 ng/ml）（P = 0.009）。患骨关节炎犬血清中TIMP-2的浓度（116.2±43.1 ng/ml）与对照犬（95.1±94.4 ng/ml）相比无显著差异（P = 0.554）。此外，还采用磷酸化酪氨酸免疫沉淀和质谱分析法从犬滑液中分离并鉴定了α干扰素。
