一种大肠杆菌插入元件（IS2）在百日咳博德特氏菌中提供了一个功能性启动子。

An Escherichia coli insertion element (IS2) provides a functional promoter in Bordetella pertussis.

作者信息

Goyard S, Pidoux J, Ullmann A

机构信息

Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, Paris.

出版信息

Res Microbiol. 1991 Jul-Aug;142(6):633-41. doi: 10.1016/0923-2508(91)90076-m.

DOI:10.1016/0923-2508(91)90076-m

PMID:1660176

Abstract

The adenylate cyclase (cyaA) gene of Bordetella pertussis is not expressed in Escherichia coli. Using cya-lac fusions, high-expression spontaneous mutants were isolated and shown to have the insertion element IS2 in orientation II integrated into the reading frame of cyaA. Upon transfer of the IS2-activated cya-lac fusion into B. pertussis, we found that the IS2-provided promoter is as efficient in B. pertussis as it is in E. coli. These results provide evidence that an insertion element derived from the E. coli chromosome can activate gene expression in B. pertussis, a taxonomically distant organism.

摘要

百日咳博德特氏菌的腺苷酸环化酶（cyaA）基因在大肠杆菌中不表达。利用cya-lac融合体，分离出了高表达自发突变体，并显示其具有以方向II整合到cyaA阅读框中的插入元件IS2。将IS2激活的cya-lac融合体转移到百日咳博德特氏菌后，我们发现IS2提供的启动子在百日咳博德特氏菌中的效率与在大肠杆菌中的效率一样高。这些结果证明，源自大肠杆菌染色体的插入元件可以激活分类学上远缘的百日咳博德特氏菌中的基因表达。