Macicková Tatiana, Pecivová Jana, Nosál' Radomír, Holománová Dagmar
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2005 Dec;149(2):389-92. doi: 10.5507/bp.2005.065.
Activation of neutrophils induces generation of reactive oxygen species and release of granule enzymes, which not only participate in the bactericidal mechanisms of these cells, but also in possible tissue damage. We studied the effect of carvedilol (CARV) [0.1-100 micromol/l], an antihypertensive and cardiovascular drug with antioxidative properties, on superoxide generation (SO) and myeloperoxidase (MPO) release from isolated human neutrophils stimulated with fMLP, a specific receptor activator, or with PMA, a receptor bypassing stimulus. Unstimulated cells showed neither SO formation nor MPO release after preincubation with drug. CARV decreased fMLP and PMA stimulated MPO release and SO generation dose dependently. The inhibitory effect of CARV may attributed to non-specific action since its effect was not influenced by the type of stimulation. It might inhibit SO generation as well as MPO release either by membrane-operating stimulus (fMLP) or membrane bypassing activator (PMA).
中性粒细胞的激活会诱导活性氧的产生和颗粒酶的释放，这不仅参与这些细胞的杀菌机制，还可能导致组织损伤。我们研究了具有抗氧化特性的降压和心血管药物卡维地洛（CARV）[0.1 - 100微摩尔/升]对超氧化物生成（SO）和髓过氧化物酶（MPO）释放的影响，这些超氧化物和髓过氧化物酶是由特异性受体激活剂fMLP或受体旁路刺激剂PMA刺激分离的人中性粒细胞产生的。未刺激的细胞在与药物预孵育后既不显示SO形成也不显示MPO释放。CARV剂量依赖性地降低了fMLP和PMA刺激的MPO释放和SO生成。CARV的抑制作用可能归因于非特异性作用，因为其作用不受刺激类型的影响。它可能通过膜操作刺激（fMLP）或膜旁路激活剂（PMA）抑制SO生成以及MPO释放。
