酵母磷脂酰丝氨酸合酶基因PSS调控区的功能分析
Functional analysis of the regulatory region of the yeast phosphatidylserine synthase gene, PSS.
作者信息
Kodaki T, Nikawa J, Hosaka K, Yamashita S
机构信息
Department of Biochemistry, Gunma University School of Medicine, Maebashi, Japan.
出版信息
J Bacteriol. 1991 Dec;173(24):7992-5. doi: 10.1128/jb.173.24.7992-7995.1991.
Abstract
The upstream region of the PSS gene contains three positive cis-acting elements, upstream activation sequences 1 and 2 (UAS1 and UAS2) and a TATA box. The 5' end of UAS1 occurs between positions -239 and -209, and that of UAS2 is between positions -172 and -164. UAS2 contains 5'-TTCACATG-3' as a core sequence at positions -161 to -154. Mutational analysis revealed that this octamer is responsible for the control of PSS expression by inositol and choline. The TATA box is located at positions -112 to -108. In addition, PSS contains a negative cis-acting sequence between UAS2 and the TATA box.
摘要
PSS基因的上游区域包含三个正向顺式作用元件，即上游激活序列1和2（UAS1和UAS2）以及一个TATA框。UAS1的5'端位于-239至-209位之间，UAS2的5'端位于-172至-164位之间。UAS2在-161至-154位包含5'-TTCACATG-3'作为核心序列。突变分析表明，这个八聚体负责肌醇和胆碱对PSS表达的调控。TATA框位于-112至-108位。此外，PSS在UAS2和TATA框之间包含一个负向顺式作用序列。
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