Oner Hakan, Oner Jale, Demir Ramazan
Department of Histology and Embryology, Faculty of Veterinary Medicine, Akdeniz University, 15100 Burdur, Turkey.
J Morphol. 2006 Jul;267(7):822-30. doi: 10.1002/jmor.10449.
The purpose of this study was to demonstrate the expression of nidogen-1 and nidogen-2 and their possible role in decidualization and implantation events during early pregnancy in rats. The tissue samples were examined from pregnant animals between gestational days 1-8 using immunocytochemistry. The uterine luminal epithelium, the glandular epithelium, and the myometrial smooth muscle cells stained strongly from gestational days 1-8 with both nidogen antibodies. At day 4 the decidual reaction areas began to appear in the stromal matrix and immunostaining of both nidogens revealed that the basement membrane of the surface epithelium was discontinuous. The differentiation of stromal cells into decidual cells was seen at gestational day 5 and both nidogens were weakly expressed in the decidualizing cells. At day 6, nidogen-2 immunoreactivity was higher in the primary decidual cells close to the embryo than nidogen-1, and during development of the decidual tissue both nidogens appeared in the endometrial stromal cells. At day 7, while expression of both nidogens declined in the primary decidual cells, their expression was markedly observed in the secondary decidual cells close to the myometrium. At day 8, expression of both nidogens was also observed to increase in the primary decidual cells. While nidogen-2 expression was seen in the parietal endoderm and primary ectoderm of the rat embryos at this developmental stage, nidogen-1 expression was only detected in the parietal endoderm. These results indicate that nidogen-1 and nidogen-2 could play important roles during embryogenesis, decidualization, and implantation in the endometrium of rat uterus.
本研究的目的是证明巢蛋白-1和巢蛋白-2的表达及其在大鼠早期妊娠蜕膜化和着床过程中的可能作用。使用免疫细胞化学方法对妊娠第1至8天的妊娠动物组织样本进行检查。从妊娠第1至8天,子宫腔上皮、腺上皮和子宫肌层平滑肌细胞用两种巢蛋白抗体染色均呈强阳性。在第4天,蜕膜反应区开始出现在基质中,两种巢蛋白的免疫染色显示表面上皮的基底膜不连续。在妊娠第5天可见基质细胞分化为蜕膜细胞,两种巢蛋白在蜕膜化细胞中均弱表达。在第6天,靠近胚胎的初级蜕膜细胞中巢蛋白-2的免疫反应性高于巢蛋白-1,在蜕膜组织发育过程中,两种巢蛋白均出现在子宫内膜基质细胞中。在第7天,虽然两种巢蛋白在初级蜕膜细胞中的表达下降,但在靠近子宫肌层的次级蜕膜细胞中明显观察到它们的表达。在第8天,两种巢蛋白在初级蜕膜细胞中的表达也被观察到增加。在这个发育阶段,虽然在大鼠胚胎的壁内胚层和初级外胚层中可见巢蛋白-2的表达,但仅在壁内胚层中检测到巢蛋白-1的表达。这些结果表明,巢蛋白-1和巢蛋白-2在大鼠子宫内膜的胚胎发生、蜕膜化和着床过程中可能发挥重要作用。