Sotobori Tsukasa, Ueda Takafumi, Oji Yusuke, Naka Norifumi, Araki Nobuhito, Myoui Akira, Sugiyama Haruo, Yoshikawa Hideki
Department of Orthopedics, Osaka University Graduate School of Medicine, Yamada-oka, Suita, Japan.
Cancer. 2006 May 15;106(10):2233-40. doi: 10.1002/cncr.21861.
There have been several recent reports that Wilms tumor gene (WT1) mRNA is overexpressed in many types of neoplasms, and those results suggested that WT1 has oncogenic properties. The objective of the current study was to evaluate the prognostic significance of WT1 mRNA expression in patients with soft tissue sarcoma.
Levels of WT1 mRNA expression were examined by quantitative, real-time reverse transcriptase-polymerase chain reaction analysis in frozen tissue samples from 52 patients with soft tissue sarcoma. Various clinicopathologic factors were analyzed along with the disease-specific survival rate for correlations with WT1 mRNA expression levels.
The levels of WT1 mRNA expression in a variety of soft tissue sarcomas were significantly greater compared with the levels in normal soft tissue samples (P = .0212). No significant correlation was observed between the level of WT1 mRNA expression and clinicopathologic factors, including gender, age, primary tumor site, tumor depth, tumor size, histologic grade, and distant metastasis at initial presentation. The disease-specific survival rate for patients with high WT1 mRNA expression levels was found significantly poorer compared with the rate for patients with low WT1 mRNA expression levels (P = .0182). Moreover, multivariate analysis indicated that a high WT1 mRNA expression level was an independent, adverse prognostic factor for disease-specific survival (hazards ratio, 2.6; P = .0488).
WT1 mRNA expression level can serve as a potent prognostic indicator in soft tissue sarcoma patients.
最近有几份报告称，肾母细胞瘤基因（WT1）mRNA在多种肿瘤类型中过度表达，这些结果表明WT1具有致癌特性。本研究的目的是评估WT1 mRNA表达在软组织肉瘤患者中的预后意义。
通过定量实时逆转录聚合酶链反应分析，检测了52例软组织肉瘤患者冷冻组织样本中WT1 mRNA的表达水平。分析了各种临床病理因素以及疾病特异性生存率与WT1 mRNA表达水平的相关性。
与正常软组织样本相比，多种软组织肉瘤中WT1 mRNA的表达水平显著更高（P = 0.0212）。未观察到WT1 mRNA表达水平与临床病理因素之间存在显著相关性，这些因素包括性别、年龄、原发肿瘤部位、肿瘤深度、肿瘤大小、组织学分级以及初次就诊时的远处转移情况。与WT1 mRNA表达水平低的患者相比，WT1 mRNA表达水平高的患者的疾病特异性生存率显著更低（P = 0.0182）。此外，多因素分析表明，WT1 mRNA表达水平高是疾病特异性生存的一个独立不良预后因素（风险比，2.6；P = 0.0488）。
WT1 mRNA表达水平可作为软组织肉瘤患者的有力预后指标。
