Chen B, Butlin R K, Pedro P M, Wang X Z, Harbach R E
School of Biology, The University of Leeds, Leeds, UK.
Med Vet Entomol. 2006 Mar;20(1):33-43. doi: 10.1111/j.1365-2915.2006.00604.x.
Species of the Anopheles fluviatilis James and Anopheles minimus complexes (Diptera: Culicidae) are difficult to distinguish morphologically. Members of the two complexes have been confused and, consequently, their distributions and roles in malaria transmission are uncertain. We identified numerous mosquitoes from China, Thailand, Vietnam, India, Nepal, Pakistan and Iran by single-strand conformation polymorphism (SSCP) and/or sequencing, and analysed the variation in the 28S D3 region of ribosomal DNA for members of the Minimus Subgroup and also the internal transcribed spacer region 2 (ITS2) for members of the An. fluviatilis complex. The D3 region is highly conserved between taxa and therefore could serve as a standard for molecular diagnosis of the subgroup members. D3 sequence, bionomics and malaria transmission data provide further evidence that An. fluviatilis S in India is conspecific with An. minimus C in South-east Asia. An. fluviatilis T has three ITS2 haplotypes (designated T1, T2 and Y) and its distribution in India, Nepal, Pakistan and Iran is confirmed. An. fluviatilis U is well defined on cytogenetic grounds in Uttar Pradesh, India, but is very close to An. fluviatilis T and the two species may hybridize in some regions. Variant ITS2 sequences suggest the possible existence of two additional taxa within the An. fluviatilis complex, one in Iran and another in India, provisionally designated An. fluviatilis forms V and X, respectively. The distributions of members of the An. fluviatilis and An. minimus complexes in south-central Asia are summarized.
溪流按蚊詹姆斯种和微小按蚊复合组（双翅目：蚊科）的种类在形态上难以区分。这两个复合组的成员一直存在混淆，因此它们在疟疾传播中的分布和作用尚不确定。我们通过单链构象多态性（SSCP）和/或测序鉴定了来自中国、泰国、越南、印度、尼泊尔、巴基斯坦和伊朗的大量蚊子，并分析了微小按蚊亚组成员核糖体DNA的28S D3区域的变异，以及溪流按蚊复合组成员的内部转录间隔区2（ITS2）。D3区域在不同分类单元之间高度保守，因此可作为该亚组成员分子诊断的标准。D3序列、生物学特性和疟疾传播数据提供了进一步的证据，表明印度的溪流按蚊S与东南亚的微小按蚊C是同种。溪流按蚊T有三种ITS2单倍型（命名为T1、T2和Y），并证实了其在印度、尼泊尔、巴基斯坦和伊朗的分布。在印度北方邦，溪流按蚊U在细胞遗传学基础上定义明确，但与溪流按蚊T非常接近，这两个物种可能在某些地区杂交。ITS2序列变异表明，溪流按蚊复合组内可能还存在另外两个分类单元，一个在伊朗，另一个在印度，暂分别命名为溪流按蚊V型和X型。总结了溪流按蚊和微小按蚊复合组成员在亚洲中南部的分布情况。
