α干扰素对中性粒细胞功能的影响。
Effect of interferon-alpha on neutrophil functions.
作者信息
Kasimir S, Brom J, König W
机构信息
AG Infektabwehrmechanismen, Med. Mikrobiologie und Immunologie, Ruhr Universität Bochum, Germany.
出版信息
Immunology. 1991 Oct;74(2):271-8.
Incubation of neutrophils with interferon-alpha (IFN-alpha) for 5 min and subsequent stimulation with the calcium ionophore A23187 enhanced the production of oxygen radicals, while a suppression was obtained with FMLP-stimulated cells. Similar data were observed for the direct G-protein activator sodium fluoride (NaF). Incubation of the cells with IFN-alpha and subsequent stimulation with FMLP (in the presence of cytochalasin b) reduced the generation of the chemotactic active leukotriene B4 (LTB4). The metabolism of LTB4 was significantly inhibited. IFN-alpha decreased the specific binding sites for LTB4 and increased the number of binding sites for FMLP. The GTPase activity as a parameter for the activation of G-proteins was enhanced by IFN-alpha. Preincubation of the cells with IFN-alpha and subsequent stimulation with NaF increased the GTPase activity synergistically, whereas co-incubation of IFN-alpha with FMLP showed additive effects. Our results clearly demonstrate the modulatory effects of IFN-alpha on granulocyte functions with regard to the receptor-mediated signal transduction.
将中性粒细胞与α-干扰素（IFN-α）孵育5分钟，随后用钙离子载体A23187刺激，可增强氧自由基的产生，而用FMLP刺激的细胞则出现抑制现象。对于直接的G蛋白激活剂氟化钠（NaF），也观察到了类似的数据。将细胞与IFN-α孵育，随后用FMLP刺激（在细胞松弛素b存在的情况下），可减少趋化活性白三烯B4（LTB4）的生成。LTB4的代谢受到显著抑制。IFN-α减少了LTB4的特异性结合位点，并增加了FMLP的结合位点数。作为G蛋白激活参数的GTP酶活性被IFN-α增强。将细胞预先与IFN-α孵育，随后用NaF刺激，可协同增强GTP酶活性，而IFN-α与FMLP共同孵育则显示出相加效应。我们的结果清楚地证明了IFN-α在受体介导的信号转导方面对粒细胞功能的调节作用。
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