Makrai L, Kira K, Kono A, Sasaki Y, Kakuda T, Tsubaki S, Fodor L, Varga J, Taka S
Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Hungária Krt. 23-25, 1143, Budapest, Hungary.
Acta Vet Hung. 2006 Mar;54(1):11-8. doi: 10.1556/AVet.54.2006.1.2.
The plasmid profiles of virulent Rhodococcus equi strains isolated on three horse-breeding farms located in different parts of Hungary were investigated. From 49 soil samples collected on the three farms, 490 R. equi isolates (10 from each sample) were obtained and tested for the presence of 15- to 17-kDa antigens (VapA) by immunoblotting and PCR. Ninety-eight VapA-positive isolates were detected from 30 of the 49 culture-positive samples with a prevalence ranging from 13.1% to 23.2%. Of the 98 virulent isolates, 70 contained an 85-kb type I plasmid, 13 contained an 87-kb type I plasmid, and 15 contained an 85-kb type III plasmid which had been uniquely isolated from soil isolates in the United States. This study demonstrates that the virulent form of R. equi is very widespread in the soil environment of these stud farms in Hungary and the plasmid pattern is different from farm to farm.
对从匈牙利不同地区的三个养马场分离出的致病性马红球菌菌株的质粒图谱进行了研究。从这三个农场采集的49份土壤样本中，获得了490株马红球菌分离株（每个样本10株），并通过免疫印迹和PCR检测了15至17 kDa抗原（VapA）的存在。在49个培养阳性样本中的30个样本中检测到98株VapA阳性分离株，流行率在13.1%至23.2%之间。在这98株致病分离株中，70株含有85 kb的I型质粒，13株含有87 kb的I型质粒，15株含有85 kb的III型质粒，该III型质粒是在美国从土壤分离株中独特分离出来的。这项研究表明，致病性马红球菌在匈牙利这些种马场的土壤环境中非常普遍，并且不同农场的质粒图谱不同。
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