[AML1不同转录本对巨噬细胞集落刺激因子受体（M-CSF-R）基因反式激活的影响]
[The effects of different transcripts of AML1 on the transactivation of M-CSF-R gene].
作者信息
Zhang Qing, Wang Min, Xing Hai-yan, Rao Qing, Wang Jian-xiang
机构信息
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2005 Nov;26(11):641-4.
OBJECTIVE
To observe the effects of AML1A and AML1B, two splicing isoforms of AML1, on the transactivation of macrophage colony-stimulating factor receptor (M-CSF-R), and explore the mechanism of hematopoietic stem cell committed differentiation and leukemogenesis.
METHODS
The expressive plasmids of AML1A and AML1B were constructed, and co-transfected into CV-1 cells with a luciferase reporter plasmid containing M-CSF-R promoter. The transactivity of M-CSF-R promoter was assayed by luminometer.
RESULTS
AML1B exhibited a distinct transactivity to M-CSF-R promoter with a sequence-specificity and dosage-dependent manner. AML1A showed no any transactivity but antagonized the effect of AML1B, causing marked reduction of M-CSF-R expression.
CONCLUSION
An intact structure of AML1 is necessary for transactivation of M-CSF-R. AML1A may interfere with the transactivation of AML1B, and play a key role in the fine regulation of committed differentiation of hematopoietic cell.
目的
观察急性髓系白血病1（AML1）的两种剪接异构体AML1A和AML1B对巨噬细胞集落刺激因子受体（M-CSF-R）反式激活的影响，探讨造血干细胞定向分化及白血病发生的机制。
方法
构建AML1A和AML1B表达质粒，并与含M-CSF-R启动子的荧光素酶报告质粒共转染至CV-1细胞。用发光计检测M-CSF-R启动子的反式活性。
结果
AML1B对M-CSF-R启动子表现出明显的反式活性，呈序列特异性和剂量依赖性。AML1A未表现出任何反式活性，但拮抗AML1B的作用，导致M-CSF-R表达显著降低。
结论
AML1的完整结构是M-CSF-R反式激活所必需的。AML1A可能干扰AML1B的反式激活，并在造血细胞定向分化的精细调节中起关键作用。
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