Eish Mohamed Y Z Aboul, Wells Martha J M
Center for the Management, Utilization, and Protection of Water Resources, and Department of Chemistry, Tennessee Technological University, Box 5033, Cookeville, TN 38505, USA.
J Chromatogr A. 2006 May 26;1116(1-2):272-6. doi: 10.1016/j.chroma.2006.03.064. Epub 2006 Apr 18.
The significance of this research is the application of thin-layer chromatography (TLC) to fractionate well-characterized aquatic humic materials coupled with the novel evaluation of the trihalomethane formation potential (THMFP) of the fractionated materials. Disinfection by-products such as trihalomethanes (THMs) form when natural water is treated by chlorination. Nordic Aquatic and Suwannee River fulvic and humic acids, obtained from the International Humic Substances Society, were prepared at pH 6 and 9 and fractionated on silica gel plates using a mobile phase consisting of methanol and ethyl acetate (2:1, v:v). Based on retention factor (R(f)) values, three common fractions were identified in all substances examined. Additionally, other fractions were noted that were characteristic of specific humic substances. Each of the three primary fractions derived from Nordic Aquatic fulvic acid at pH 6 demonstrated the potential to contribute to formation of THMs. This research provides data to support the hypothesis that differences in the chemical structure and composition of natural organic matter (NOM) significantly affect the potential to react with chlorine to form THMs.
本研究的意义在于应用薄层色谱法（TLC）对特征明确的水生腐殖质进行分级分离，并对分级分离后的物质进行三卤甲烷生成势（THMFP）的新型评估。当天然水进行氯化处理时会形成三卤甲烷（THMs）等消毒副产物。从国际腐殖质协会获得的北欧水生腐殖酸和苏万尼河富里酸，在pH值为6和9的条件下制备，并使用由甲醇和乙酸乙酯（2:1，v:v）组成的流动相在硅胶板上进行分级分离。根据保留因子（R(f)）值，在所检测的所有物质中鉴定出三种常见馏分。此外，还发现了其他特定腐殖质特有的馏分。在pH值为6时，从北欧水生富里酸衍生出的三种主要馏分中的每一种都显示出有助于形成三卤甲烷的潜力。本研究提供的数据支持了以下假设：天然有机物（NOM）的化学结构和组成差异会显著影响与氯反应形成三卤甲烷的潜力。
