Suppr超能文献

肝素结合表皮生长因子（HB-EGF）可能通过增加着床期小鼠胚胎中玻连蛋白受体（整合素αvβ3）的表达来改善胚胎发育和着床。

Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin alphanubeta3) expression in peri-implantation mouse embryos.

作者信息

Lim Jung Jin, Lee Dong Ryul, Song Haeng-Seok, Kim Kye-Seong, Yoon Tae Ki, Gye Myung Chan, Kim Moon Kyoo

机构信息

Department of Life Science, College of Natural Science, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Korea.

出版信息

J Assist Reprod Genet. 2006 Mar;23(3):111-9. doi: 10.1007/s10815-006-9021-9. Epub 2006 Apr 19.

DOI:10.1007/s10815-006-9021-9
PMID:16622802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455038/
Abstract

PURPOSE

This study investigated the effects of HB-EGF on expression of integrin alphanubeta3 and implantation of embryos.

METHODS

Two-cell embryos were recovered and cultured with or without 10 ng/mL HB-EGF for 96h. Expression of integrin alphanubeta3 in cultured embryos was examined by real time-RT-PCR and immunofluorescence analysis; embryos were cultured with or without HB-EGF, then transferred into the uteri of pseudo-pregnant female mice in order to analyze their implantation rate.

RESULTS

HB-EGF improved embryonic hatching and outgrowth during extended culture, and up-regulated expression of integrin alphanubeta3 in both the preimplantation embryo and outgrowing blastocyst. Also, integrin alphanubeta3 subunits were localized at the pericellular borders and cell-cell contact areas. The number of successful implantation sites of transferred HB-EGF-treated embryos in the uterus was increased when compared to number of implantation sites with non-treated controls.

CONCLUSIONS

HB-EGF may improve implantation by accelerating expression of integrin alphanubeta3 in peri-implantation mouse embryos.

摘要

目的

本研究调查了肝素结合表皮生长因子（HB-EGF）对整合素αvβ3表达及胚胎着床的影响。

方法

回收二细胞胚胎，在添加或不添加10 ng/mL HB-EGF的条件下培养96小时。通过实时逆转录聚合酶链反应（real time-RT-PCR）和免疫荧光分析检测培养胚胎中整合素αvβ3的表达；胚胎在添加或不添加HB-EGF的条件下培养，然后移植到假孕雌性小鼠子宫内，以分析其着床率。

结果

HB-EGF在延长培养期间改善了胚胎孵化和生长，并上调了着床前胚胎和生长中的囊胚中整合素αvβ3的表达。此外，整合素αvβ3亚基定位于细胞周围边界和细胞-细胞接触区域。与未处理的对照组相比，移植经HB-EGF处理的胚胎后子宫内成功着床部位的数量增加。

结论

HB-EGF可能通过加速着床期小鼠胚胎中整合素αvβ3的表达来改善着床。

相似文献

1
Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin alphanubeta3) expression in peri-implantation mouse embryos.
J Assist Reprod Genet. 2006 Mar;23(3):111-9. doi: 10.1007/s10815-006-9021-9. Epub 2006 Apr 19.
2
Improvement of implantation potential in mouse blastocysts derived from IVF by combined treatment with prolactin, epidermal growth factor and 4-hydroxyestradiol.
Mol Hum Reprod. 2017 Aug 1;23(8):557-570. doi: 10.1093/molehr/gax035.
3
Epidermal growth factor-like ligands and erbB genes in the peri-implantation rabbit uterus and blastocyst.
Biol Reprod. 2001 Jun;64(6):1835-44. doi: 10.1095/biolreprod64.6.1835.
4
Heparin-binding EGF-like growth factor interacts with mouse blastocysts independently of ErbB1: a possible role for heparan sulfate proteoglycans and ErbB4 in blastocyst implantation.
Development. 1999 May;126(9):1997-2005. doi: 10.1242/dev.126.9.1997.
5
The effects of heparin-binding epidermal growth factor-like growth factor on preimplantation-embryo development and implantation in the rat.
Life Sci. 1999;64(22):1967-73. doi: 10.1016/s0024-3205(99)00128-9.
6
Lysophosphatidic acid regulates murine blastocyst development by transactivation of receptors for heparin-binding EGF-like growth factor.
Exp Cell Res. 2004 Jun 10;296(2):317-26. doi: 10.1016/j.yexcr.2004.02.006.
7
The effect of epidermal growth factor on the preimplantation development, implantation and its receptor expression in mouse embryos.
J Obstet Gynaecol Res. 1999 Apr;25(2):87-93. doi: 10.1111/j.1447-0756.1999.tb01128.x.
8
Heparin-binding EGF-like growth factor gene is induced in the mouse uterus temporally by the blastocyst solely at the site of its apposition: a possible ligand for interaction with blastocyst EGF-receptor in implantation.
Development. 1994 May;120(5):1071-83. doi: 10.1242/dev.120.5.1071.
9
Dual source and target of heparin-binding EGF-like growth factor during the onset of implantation in the hamster.
Development. 2002 Sep;129(17):4125-34. doi: 10.1242/dev.129.17.4125.
10
Possible effects of pituitary adenylate cyclase activating polypeptide (PACAP) on early embryo implantation marker HB-EGF in mouse.
Reprod Biol. 2020 Mar;20(1):9-13. doi: 10.1016/j.repbio.2020.01.005. Epub 2020 Jan 18.

引用本文的文献

1
In vitro effects of heparin-binding epidermal growth factor on adhesion stage of implantation.
Rom J Morphol Embryol. 2023 Oct-Dec;64(4):493-500. doi: 10.47162/RJME.64.4.05.
2
Uterine WNTS modulates fibronectin binding activity required for blastocyst attachment through the WNT/CA signaling pathway in mice.
Reprod Biol Endocrinol. 2023 Sep 15;21(1):85. doi: 10.1186/s12958-023-01135-0.
3
GM-CSF (granulocyte macrophage colony-stimulating factor) supplementation in culture media for women undergoing assisted reproduction.
Cochrane Database Syst Rev. 2020 Jul 16;7(7):CD013497. doi: 10.1002/14651858.CD013497.pub2.
4
Melatonin Pretreated Blastocysts along with Calcitonin Administration Improved Implantation by Upregulation of Heparin Binding-Epidermal Growth Factor Expression in Murine Endometrium.
Cell J. 2018 Jan;19(4):599-606. doi: 10.22074/cellj.2018.4737. Epub 2017 Nov 4.
5
Local and systemic factors and implantation: what is the evidence?
Fertil Steril. 2016 Apr;105(4):873-84. doi: 10.1016/j.fertnstert.2016.02.018. Epub 2016 Mar 3.
6
Assessment of α4, αv, β1 and β3 integrins expression throughout the implantation window phase in endometrium of a mouse model of polycystic ovarian syndromes.
Iran J Reprod Med. 2014 Oct;12(10):687-94.
7
Differential gene expression in the endometrium on gestation day 12 provides insight into sow prolificacy.
BMC Genomics. 2013 Jan 22;14:45. doi: 10.1186/1471-2164-14-45.
8
Function-specific intracellular signaling pathways downstream of heparin-binding EGF-like growth factor utilized by human trophoblasts.
Biol Reprod. 2010 May;82(5):921-9. doi: 10.1095/biolreprod.109.082305. Epub 2010 Feb 3.
9
Effect of nerve growth factor (NGF) on the development of preimplantation rabbit embryos in vitro.
Vet Res Commun. 2010 Jan;34(1):11-8. doi: 10.1007/s11259-009-9325-1. Epub 2009 Oct 16.
10
Diverse functions of HBEGF during pregnancy.
Mol Reprod Dev. 2009 Dec;76(12):1116-27. doi: 10.1002/mrd.21066.

本文引用的文献

1
Regulated expression of heparin-binding EGF-like growth factor (HB-EGF) in the human endometrium: a potential paracrine role during implantation.
Mol Reprod Dev. 2002 Aug;62(4):446-55. doi: 10.1002/mrd.10129.
2
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
Methods. 2001 Dec;25(4):402-8. doi: 10.1006/meth.2001.1262.
3
Regulation of peri-attachment embryo development in the golden hamster: role of growth factors.
J Reprod Immunol. 2002 Jan;53(1-2):203-13. doi: 10.1016/s0165-0378(01)00086-9.
4
Osteopontin and its receptor alphavbeta(3) integrin are coexpressed in the human endometrium during the menstrual cycle but regulated differentially.
J Clin Endocrinol Metab. 2001 Oct;86(10):4991-5000. doi: 10.1210/jcem.86.10.7906.
5
Paracrine regulators of implantation.
Baillieres Best Pract Res Clin Obstet Gynaecol. 2000 Oct;14(5):815-26. doi: 10.1053/beog.2000.0121.
6
Blockade of the alpha(v)beta(3) integrin adversely affects implantation in the mouse.
Biol Reprod. 2000 May;62(5):1285-90. doi: 10.1095/biolreprod62.5.1285.
7
The effects of heparin-binding epidermal growth factor-like growth factor on preimplantation-embryo development and implantation in the rat.
Life Sci. 1999;64(22):1967-73. doi: 10.1016/s0024-3205(99)00128-9.
8
Heparin-binding EGF-like growth factor interacts with mouse blastocysts independently of ErbB1: a possible role for heparan sulfate proteoglycans and ErbB4 in blastocyst implantation.
Development. 1999 May;126(9):1997-2005. doi: 10.1242/dev.126.9.1997.
9
erbB genes in the mouse uterus: cell-specific signaling by epidermal growth factor (EGF) family of growth factors during implantation.
Dev Biol. 1998 Dec 1;204(1):97-110. doi: 10.1006/dbio.1998.9072.
10
Heparin-binding epidermal growth factor significantly improves human blastocyst development and hatching in serum-free medium.
Hum Reprod. 1998 Jun;13(6):1645-52. doi: 10.1093/humrep/13.6.1645.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验