犬血浆和脑脊液中转甲状腺素蛋白及视黄醇结合蛋白的特性分析

Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs.

作者信息

Forterre Simone, Raila Jens, Forterre Franck, Brunnberg Leo, Schweigert Florian J

机构信息

Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Potsdam-Rehbrücke, Germany.

出版信息

Vet J. 2006 May;171(3):451-5. doi: 10.1016/j.tvjl.2004.11.017.

DOI:10.1016/j.tvjl.2004.11.017

PMID:16624711

Abstract

The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS-PAGE. Vitamin A was determined by high performance liquid chromatography. RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10-100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.

摘要

本研究的目的是调查犬血浆和脑脊液（CSF）中维生素A、转甲状腺素蛋白（TTR）和视黄醇结合蛋白（RBP）浓度的差异。采用酶联免疫吸附测定（ELISA）检测RBP，并在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离后，通过蛋白质免疫印迹分析检测RBP和TTR。采用高效液相色谱法测定维生素A。在所有样本中均检测到RBP、TTR以及维生素A，但与血浆相比，脑脊液中的浓度要低得多。犬血浆中的RBP分子量与人的相似，而犬TTR的分子量较低。血浆和脑脊液的比较表明，脑脊液中RBP和TTR的分子量均较低。在脑脊液中，RBP和视黄醇的浓度比血浆低10至100倍。在17个样本中的5个样本中，视黄酯仅以微量存在。总之，与人类相比，犬脑脊液中维生素A转运蛋白的质量和数量均存在显著差异。