患有肺嗜酸性粒细胞增多症的犬支气管肺泡灌洗液中，层粘连蛋白-5γ2链片段水平升高。

Elevated levels of fragmented laminin-5 gamma2-chain in bronchoalveolar lavage fluid from dogs with pulmonary eosinophilia.

作者信息

Rajamäki Minna M, Järvinen Anna-Kaisa, Sorsa Timo A, Tervahartiala Taina I, Maisi Päivi S

机构信息

Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, 00014 University of Helsinki, P.O. Box 57, Hämeentie 57, Helsinki, Finland.

出版信息

Vet J. 2006 May;171(3):562-5. doi: 10.1016/j.tvjl.2004.11.018.

DOI:10.1016/j.tvjl.2004.11.018

PMID:16624727

Abstract

Inflammation causes epithelial cell sloughing and basement membrane (BM) exposure in canine pulmonary eosinophilia (PE), leading to degradation of the epithelial cell attachment component, laminin-5 gamma2-chain, into small molecular weight fragments. The subsidence of inflammation after treatment down-regulates degradation. Laminin-5 gamma2-chain levels and molecular forms in bronchoalveolar lavage fluid (BALF) were analysed semiquantitatively by Western immunoblotting to compare PE affected (n=20) and healthy dogs (n=16) as well as PE dogs (n=6) before and after corticosteroid treatment. PE dogs expressed significantly elevated levels of total (P<0.01), 36 kDa (P<0.05) and 53 kDa (P<0.05) laminin-5 gamma2-fragments. The 36 Da fragment decreased significantly (P<0.05) after treatment. The laminin-5 gamma2-chain degradation products may be linked to epithelial cell sloughing and BM exposure or healing.

摘要

炎症会导致犬类肺嗜酸性粒细胞增多症（PE）中上皮细胞脱落和基底膜（BM）暴露，进而致使上皮细胞黏附成分层粘连蛋白-5γ2链降解为小分子量片段。治疗后炎症消退会下调这种降解过程。通过蛋白质免疫印迹法对支气管肺泡灌洗液（BALF）中层粘连蛋白-5γ2链的水平和分子形式进行半定量分析，以比较20只患PE的犬、16只健康犬以及6只接受皮质类固醇治疗前后的患PE犬。患PE的犬表达的层粘连蛋白-5γ2片段总量（P<0.01）、36 kDa（P<0.05）和5³ kDa（P<0.05）显著升高。治疗后36 Da片段显著减少（P<0.05）。层粘连蛋白-5γ2链降解产物可能与上皮细胞脱落、BM暴露或愈合有关。