Wu Yuan-dong, Tan Wan-long, Xie Yi, Yu Zhao-cun, Zhao Guo-zhi
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Apr;26(4):436-40.
To label a human bladder cancer cell line and establish a novel human bladder cancer mouse model.
T-24 cells, a human bladder transitional cell carcinoma cell line, were transfected with GFP plasmid to screen stable GFP-expressing clones. The latter were implanted into the wall of the bladder or the subcutaneous tissue of the neck of nude mice. The growth, invasion, and metastasis of the implanted tumor were observed and evaluated with whole-body optical imaging system. The findings were compared with those of HE staining on routine paraffin sections.
GFP-labeled tumor cells displayed green fluorescence under fluorescent microscopy and showed stable GFP expression in vitro and in vivo. One week after in situ transplantation of 5 x 10(5) T24 cells, the new bladder cancer was observed and evaluated under whole-body optical imaging system. Two weeks later, the new bladder tumor could be palpated, and 4 weeks later, metastasis to regional drainage lymph nodes in the pelvic and retroperitoneal lymph nodes occurred. The growth and metastasis of the implant bladder tumor were easily observed and accurately evaluated by fluorescent microscope.
GFP-labeled tumor cells display green fluorescence under fluorescent microscopy and show stable GFP expression. GFP-labeled T-24 cells and the novel human bladder cancer model described hereby provide a simple and reliable means for studying human bladder cancer in vivo.
标记人膀胱癌细胞系并建立新型人膀胱癌小鼠模型。
将人膀胱移行细胞癌细胞系T-24细胞用绿色荧光蛋白（GFP）质粒转染，筛选出稳定表达GFP的克隆。将后者植入裸鼠膀胱壁或颈部皮下组织。用全身光学成像系统观察和评估植入肿瘤的生长、侵袭和转移情况。将结果与常规石蜡切片苏木精-伊红（HE）染色结果进行比较。
GFP标记的肿瘤细胞在荧光显微镜下呈现绿色荧光，在体内外均显示稳定的GFP表达。原位移植5×10⁵个T24细胞1周后，通过全身光学成像系统观察并评估新形成的膀胱癌。2周后可触及新形成的膀胱肿瘤，4周后发生转移至盆腔和腹膜后区域引流淋巴结。植入的膀胱肿瘤的生长和转移情况通过荧光显微镜易于观察且能准确评估。
GFP标记的肿瘤细胞在荧光显微镜下呈现绿色荧光并显示稳定的GFP表达。GFP标记的T-24细胞及本文所述的新型人膀胱癌模型为体内研究人膀胱癌提供了一种简单可靠的方法。
