非包膜型流感嗜血杆菌临床分离株中菌毛基因hifA的出现情况。

Occurrence of the Fimbria Gene hifA in clinical isolates of nonencapsulated Haemophilus influenzae.

作者信息

Nakamura Masahiko, Asaka Toshiyuki, Kirita Akiko, Miyazaki Hatsumi, Senda Yasuko, Fujita Shin-Ichi, Fukushima Ritsuko, Watanabe Kishichiro, Karasawa Tadahiro, Kawahara Ei, Shimura Satoshi, Yamagishi Takayoshi

机构信息

Department of Clinical Laboratory Science, Division of Health Sciences, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan.

出版信息

Microbiol Immunol. 2006;50(4):327-9. doi: 10.1111/j.1348-0421.2006.tb03800.x.

DOI:10.1111/j.1348-0421.2006.tb03800.x

PMID:16625054

Abstract

The adherence of Haemophilus influenzae to epithelial cells plays a crucial role in infections. However, little is known about the occurrence of fimbriae. In this study, we examined the distribution of the fimbria gene (hifA) by PCR among 167 H. influenzae strains isolated from patients with respiratory infections. Almost all (163; 98%) of the isolates were nonencapsulated strains. The carriage rate of hifA by the nonencapsulated strains was 18.4%. Electron microscopy showed that fimbriae were abundantly present on the cell surface of hifA-positive strains tested. Only four (2.4%) isolates were encapsulated, all of which were type b and did not possess hifA. The present work suggests that fimbriae may play a considerable role as adhesins in nonencapsulated H. influenzae strains.

摘要

流感嗜血杆菌对上皮细胞的黏附在感染过程中起着关键作用。然而，关于菌毛的存在情况却知之甚少。在本研究中，我们通过聚合酶链反应（PCR）检测了从呼吸道感染患者中分离出的167株流感嗜血杆菌菌株中菌毛基因（hifA）的分布情况。几乎所有（163株；98%）分离株均为非包膜菌株。非包膜菌株中hifA的携带率为18.4%。电子显微镜显示，在所检测的hifA阳性菌株的细胞表面大量存在菌毛。仅4株（2.4%）分离株为包膜菌株，且均为b型，不具有hifA。目前的研究表明，菌毛可能作为黏附素在非包膜流感嗜血杆菌菌株中发挥重要作用。