毒素相关的人单磷酸腺苷 - 核糖基转移酶3在人睾丸中的表达。

Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes.

作者信息

Friedrich Maik, Grahnert Andreas, Paasch Uwe, Tannapfel Andrea, Koch-Nolte Friedrich, Hauschildt Sunna

机构信息

Institute of Biology II, Deptartment of Immunobiology, University of Leipzig, Talstrasse 33, D-04103 Leipzig, Germany.

出版信息

Asian J Androl. 2006 May;8(3):281-7. doi: 10.1111/j.1745-7262.2006.00125.x.

DOI:10.1111/j.1745-7262.2006.00125.x

PMID:16625277

Abstract

AIM

To investigate wether the corresponding protein of mono-ADP-ribosyltransferase 3 (ART3) mRNA is expressed in human testes and, if so, whether the expression is cell type-specific.

METHODS

ART3 mRNA was determined in human testes and sperm by reverse transcription-polymerase chain reaction (RT-PCR). The glycosyl-phosphatidylinositol linkage of ART3 was shown by treating ART3-transfected HEK-293-T cells with phospholipase C. Fluorescent activated cell sorter (FACS)-analyses were used to detect ART3 on mature spermatozoa and immunohistological studies to detect the protein in testes.

RESULTS

ART3 protein was shown to be present in testes. It was found on spermatocytes only. It was absent from spermatogonia, spermatids and spermatozoa. The absence of ART3 from spermatozoa was confirmed by FACS-analysis. ART3 protein was detected neither within a seminoma nor on Leydig cells.

CONCLUSION

Here we show for the first time that ART3 protein is expressed in testes in particular on spermatocytes, indicating that ART3 exerts a specific function only required at a particular stage of spermatogenesis.

摘要

目的

研究单 ADP - 核糖基转移酶 3（ART3）mRNA 的相应蛋白是否在人类睾丸中表达，若表达，其表达是否具有细胞类型特异性。

方法

采用逆转录 - 聚合酶链反应（RT - PCR）检测人类睾丸和精子中的 ART3 mRNA。通过用磷脂酶 C 处理 ART3 转染的 HEK - 293 - T 细胞来显示 ART3 的糖基磷脂酰肌醇连接。使用荧光激活细胞分选仪（FACS）分析来检测成熟精子上的 ART3，并通过免疫组织学研究检测睾丸中的该蛋白。