Booij Jan, de Bruin Kora, Gunning W Boudewijn
Department of Nuclear Medicine, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands.
Nucl Med Biol. 2006 Apr;33(3):409-11. doi: 10.1016/j.nucmedbio.2005.12.007. Epub 2006 Mar 9.
In recent years, several PET and SPECT studies have shown loss of striatal dopamine transporter (DAT) binding in amphetamine (AMPH) users. However, the use of DAT SPECT tracers to detect AMPH-induced changes in DAT binding has not been validated. We therefore examined if repeated administration of D-AMPH or methamphetamine (METH) may induce loss of binding to striatal DATs in rats by using an experimental biodistribution study design and a SPECT tracer for the DAT ([123I]FP-CIT).
Groups of male rats (n = 10 per group) were treated with D-AMPH (10 mg/kg body weight), METH (10 mg/kg body weight), or saline, twice a day for 5 consecutive days. Five days later, [123I]FP-CIT was injected intravenously, and 2 h later, the rats were sacrificed and radioactivity was assayed.
In d-AMPH but not METH-treated rats, striatal [123I]FP-CIT uptake was significantly lower (approximately 17%) than in the control group.
These data show that [123I]FP-CIT can be used to detect AMPH-induced changes in DAT binding and may validate the use of DAT radiotracers to study AMPH-induced changes in striatal DAT binding in vivo.
近年来，多项正电子发射断层扫描（PET）和单光子发射计算机断层扫描（SPECT）研究表明，苯丙胺（AMPH）使用者纹状体多巴胺转运体（DAT）结合减少。然而，使用DAT SPECT示踪剂检测AMPH诱导的DAT结合变化尚未得到验证。因此，我们通过实验性生物分布研究设计和DAT的SPECT示踪剂（[123I]FP-CIT），研究了重复给予D-AMPH或甲基苯丙胺（METH）是否会导致大鼠纹状体DAT结合丧失。
将雄性大鼠分组（每组n = 10），分别用D-AMPH（10 mg/kg体重）、METH（10 mg/kg体重）或生理盐水处理，每天两次，连续5天。5天后，静脉注射[123I]FP-CIT，2小时后处死大鼠并测定放射性。
在D-AMPH处理而非METH处理的大鼠中，纹状体[123I]FP-CIT摄取显著低于对照组（约17%）。
这些数据表明，[123I]FP-CIT可用于检测AMPH诱导的DAT结合变化，并可能验证使用DAT放射性示踪剂在体内研究AMPH诱导的纹状体DAT结合变化的可行性。
