Cofiel Luciana Pereira do Vale, Mattioli Rosana
Laboratory of Neuroscience, Universidade Federal de São Carlos, São Carlos, SP-Brazil.
Prog Neuropsychopharmacol Biol Psychiatry. 2006 Sep 30;30(7):1246-50. doi: 10.1016/j.pnpbp.2006.03.017. Epub 2006 Apr 24.
This study investigated the involvement of H(1) and H(2) histaminegic receptors on the acquisition of a new task in Carassius auratus by using an inhibitory avoidance paradigm in which the animals had to learn to avoid an aversive stimulus. Before training, the fish received injections of H(2) antagonist zolantidine at a dose of 20 mg/kg, or H(1) antagonist chlorpheniramine at a dose of 4 or 16 mg/kg. Control animals were injected with distilled water. A facilitatory effect of chlorpheniramine was observed at the dose of 16 mg/kg. On the other hand, the administration of 20 mg/kg of zolantidine inhibited acquisition. Place preference conditioning was used to observe the aversive or reinforcing effects of the drugs, which could interfere with the inhibitory avoidance procedure; however, no effects were observed. Thus, it can be suggested that both receptors, H(1) and H(2), are involved in the acquisition of a new task in this species.
本研究通过使用抑制性回避范式来探究H(1)和H(2)组胺能受体在鲫鱼获取新任务过程中的作用，在此范式中动物必须学会避免厌恶刺激。训练前，鱼被注射20mg/kg剂量的H(2)拮抗剂佐兰替丁，或4mg/kg或16mg/kg剂量的H(1)拮抗剂氯苯那敏。对照动物注射蒸馏水。在16mg/kg剂量时观察到氯苯那敏有促进作用。另一方面，给予20mg/kg佐兰替丁会抑制学习获取。采用位置偏爱条件反射来观察药物的厌恶或强化作用，其可能干扰抑制性回避程序；然而，未观察到任何作用。因此，可以认为H(1)和H(2)这两种受体均参与了该物种获取新任务的过程。
