Xiang Jin, Fan Jun-Bao, Chen Nan, Chen Jie, Liang Yi
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Colloids Surf B Biointerfaces. 2006 May 1;49(2):175-80. doi: 10.1016/j.colsurfb.2006.03.015. Epub 2006 Mar 22.
The interactions between Trichoderma reesei cellulase and an anionic surfactant, sodium dodecyl sulfate (SDS), at critical micelle concentration level have been investigated using isothermal titration calorimetry, fluorescence spectroscopy, and circular dichroism. SDS micelles have dual interactions with cellulase: electrostatic at first and then hydrophobic interactions. When the concentration of SDS is smaller than 45.0mM, SDS micelles cause a partial loss in the hydrolytic activity together with a steep decrease in the alpha-helical content of cellulase. With further increasing the concentration of SDS, however, a re-formation of the alpha-helical structure and a partial recovery of the hydrolytic activity of cellulase induced by SDS micelles are observed. Taken together, these results indicate that SDS micelles exert dual effects on cellulase through binding as both a denaturant and a recovery reagent.
利用等温滴定量热法、荧光光谱法和圆二色性，研究了里氏木霉纤维素酶与临界胶束浓度水平的阴离子表面活性剂十二烷基硫酸钠（SDS）之间的相互作用。SDS胶束与纤维素酶有双重相互作用：首先是静电相互作用，然后是疏水相互作用。当SDS浓度小于45.0mM时，SDS胶束会导致纤维素酶的水解活性部分丧失，同时纤维素酶的α-螺旋含量急剧下降。然而，随着SDS浓度的进一步增加，观察到由SDS胶束诱导的纤维素酶α-螺旋结构的重新形成和水解活性的部分恢复。综上所述，这些结果表明SDS胶束通过作为变性剂和恢复剂结合，对纤维素酶发挥双重作用。
