Hashi Akihiko, Xu Jia-Ying, Kondo Tetsuo, Hashi Keiko, Yuminamochi Tsutomu, Nara Masatoshi, Murata Shin-Ichi, Katoh Ryohei, Hoshi Kazuhiko
Department of Obstetrics and Gynecology, and Clinical Laboratory, Faculty of Medicine, University of Yamanashi, Yamanashi, Japan.
Int J Gynecol Pathol. 2006 Apr;25(2):187-94. doi: 10.1097/01.pgp.0000179612.63085.70.
A high rate of human papillomavirus (HPV) infection has been reported in cervical cancer and precancerous lesions. Many studies also have shown that p16INK4a overexpression is of diagnostic value for high-risk HPV-related cervical cancer and precursors. Lobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. There is one report about HPV infection and few studies on p16INK4a expression in LEGH. Therefore, we 1) detected HPV infection and examined p16INK4a expression and 2) observed the relationship between HPV and p16INK4a overexpression in LEGH. The immunohistochemical expression of p16INK4a was studied in 24 cases of LEGH. HPV DNA was also evaluated in these cases using a polymerase chain reaction technique. Strong (++) p16INK4a immunoreactivity was observed in 10 (41.7%) of the 24 LEGH cases; a moderate (+) pattern was observed in 9 (37.5%) cases; a weak (+) pattern was observed in 2 (8.3%) cases; and the remaining 3 (12.5%) cases showed negative expression. Overall, p16INK4a overexpression was seen in 87.5% of the cases (21/24). HPV DNA was not detected in any of the 24 LEGH cases. These results suggest that p16INK4a overexpression is independent of HPV infection in LEGH.
据报道，宫颈癌及癌前病变中人类乳头瘤病毒（HPV）感染率较高。许多研究还表明，p16INK4a过表达对高危HPV相关宫颈癌及癌前病变具有诊断价值。宫颈小叶内腺增生（LEGH）是一种罕见的子宫颈病变。有一篇关于HPV感染的报道，而关于LEGH中p16INK4a表达的研究较少。因此，我们1）检测HPV感染并检测p16INK4a表达，2）观察LEGH中HPV与p16INK4a过表达之间的关系。对24例LEGH病例进行了p16INK4a的免疫组化表达研究。同时采用聚合酶链反应技术对这些病例中的HPV DNA进行评估。在24例LEGH病例中，10例（41.7%）观察到强（++）p16INK4a免疫反应性；9例（37.5%）观察到中度（+）模式；2例（8.3%）观察到弱（+）模式；其余3例（12.5%）显示阴性表达。总体而言，87.5%的病例（21/24）出现p16INK4a过表达。24例LEGH病例中均未检测到HPV DNA。这些结果表明，LEGH中p16INK4a过表达与HPV感染无关。
