Beuth J, Schneider H, Ko H L
Institute of Naturopathy, University of Koeln, 50931 Koeln, Germany.
In Vivo. 2006 Mar-Apr;20(2):247-51.
An aqueous plant extract from Azadirachta indica and its chromatographic fraction F1 (neem extract) exerted immunomodulating and antimetastatic activities in BALB/ c-mice. Regular subcutaneous administration of neem extract yielded significantly increased spleen weight and significant enhancement of peritoneal macrophage activity in the chemiluminescence assay, and activation marker CD-44 expression. The thymus weight and thymocyte counts did not show significant differences in the control and neem extract-treated groups, however, determination of peripheral blood cells revealed significant up-regulations of leukocyte subsets, the lymphocytes and monocytes. Flow cytometric analaysis of lymphocyte supopulations documented increased counts of CD-4 and CD-8 cells and an inreased activation marker expression on lymphocytes (CD-25) and monocytes (MAC-3) in neem-treated mice compared to the control animals. To evaluate the antimetastatic activity of neem extract, sarcoma L-1 cells and lymphosarcoma RAW cells were intravenously inoculated into BALB/c-mice. In these model systems the number of experimental lung and liver metastases decreased relevantly, however, biometrically non-significantly in neem extract-treated animals, as compared to the control mice which received injections of saline solutions. Neem extract can be regarded as an immunomodulating and antimetastatic substance which holds promise for further experimental and clinical investigation.
印楝的水提物及其色谱级分F1（印楝提取物）对BALB/c小鼠具有免疫调节和抗转移活性。定期皮下注射印楝提取物可使脾脏重量显著增加，在化学发光测定中腹膜巨噬细胞活性显著增强，且激活标志物CD-44表达增加。对照组和印楝提取物处理组的胸腺重量和胸腺细胞计数无显著差异，然而，外周血细胞测定显示白细胞亚群、淋巴细胞和单核细胞显著上调。与对照动物相比，对淋巴细胞亚群进行流式细胞术分析表明，印楝处理小鼠的CD-4和CD-8细胞计数增加，淋巴细胞（CD-25）和单核细胞（MAC-3）上的激活标志物表达增加。为评估印楝提取物的抗转移活性，将肉瘤L-1细胞和淋巴肉瘤RAW细胞静脉注射到BALB/c小鼠体内。在这些模型系统中，与接受生理盐水注射的对照小鼠相比，印楝提取物处理动物的实验性肺和肝转移瘤数量有所减少，但从生物统计学角度来看差异不显著。印楝提取物可被视为一种免疫调节和抗转移物质，有望用于进一步的实验和临床研究。
