Lee JongUn, Yoo Kwangjay, Kim Soo Wan, Jung Kyoon Ho, Ma Seong Kwon, Lee Yeon Kyung, Kim Wan-Young, Kim Jin, Choi Ki Chul
Department of Physiology, Chonnam National University Medical School, Gwangju, Korea.
Nephron Physiol. 2006;103(4):p170-8. doi: 10.1159/000092918. Epub 2006 Apr 24.
A neural mechanism regulating aquaporin (AQP) water channels in the kidney was investigated.
Male Sprague-Dawley rats were used. Renal denervation was induced by painting the renal vessels with 10% phenol. The expression of AQP1-4 proteins was determined in the denervated and contralateral kidneys. The expression was also examined in rats which were renally denervated and subjected to water restriction or deoxycorticosterone acetate (DOCA)-salt treatment.
Following the unilateral denervation, tissue contents of norepinephrine were significantly decreased in the denervated kidney, while increased in the contralateral kidney. Accordingly, the expression of AQP1-4 proteins was decreased by 15-40% in the denervated kidney, and increased by 30-50% in the contralateral kidney. Immunohistochemistry of AQP2 confirmed its decreases in the denervated kidney and increases in the contralateral kidney. In bilaterally denervated rats, the urine flow increased along with decreased osmolarity. The water restriction increased the expression of AQP channels, however, the magnitude of which was lower in the denervated than in the contralateral kidney. Renal denervation decreased the degree of DOCA-salt hypertension, along with lower expression of AQP channels.
It is suggested that the sympathetic nerve should play a specific excitatory role in the regulation of AQP channels in the kidney.
研究调节肾脏水通道蛋白（AQP）水通道的神经机制。
使用雄性Sprague-Dawley大鼠。通过用10%苯酚涂抹肾血管诱导肾去神经支配。测定去神经支配侧和对侧肾脏中AQP1 - 4蛋白的表达。还在肾去神经支配并进行限水或醋酸脱氧皮质酮（DOCA）-盐处理的大鼠中检测该表达。
单侧去神经支配后，去神经支配侧肾脏中去甲肾上腺素的组织含量显著降低，而对侧肾脏中则升高。相应地，去神经支配侧肾脏中AQP1 - 4蛋白的表达降低了15 - 40%，对侧肾脏中则升高了30 - 50%。AQP2的免疫组织化学证实其在去神经支配侧肾脏中减少，在对侧肾脏中增加。在双侧去神经支配的大鼠中，尿流量增加，同时渗透压降低。限水增加了AQP通道的表达，然而，去神经支配侧的增加幅度低于对侧肾脏。肾去神经支配降低了DOCA-盐高血压的程度，同时AQP通道的表达也降低。
提示交感神经在调节肾脏AQP通道中应发挥特定的兴奋作用。
