[血小板活化检测的进展：聚焦于血小板衍生微粒检测]

[Advancement of platelet activation measurement: focusing on platelet-derived microparticle measurement].

作者信息

Hayasaka Koji, Moriyama Takanori, Chiba Hitoshi, Matsuno Kazuhiko

机构信息

Department of Laboratory Medicine, Hokkaido University Hospital, Sapporo 060-8648.

出版信息

Rinsho Byori. 2006 Mar;54(3):250-5.

PMID:16637573

Abstract

The assessment of the extent of platelet activation in various thrombotic disorders is important to detect thrombotic tendencies and monitor antiplatelet therapy. Various methods (platelet factor 4: PF4, beta-thromboglobulin: beta-TG, CD62P, PAC-1, etc.) are used as markers of platelet activation. Platelet-derived microparticles (PDMP) are submicroscopic vesicles released by platelets during activation, and are used as a marker of platelet activation. PDMP are usually determined by flow cytometry. An enzyme-linked immunosorbent assay (ELISA) method in which PDMP are detected with the antibody of CD42a and CD42b, was recently reported. The PDMP sample for ELISA can be preserved, and standardization is also easy. It is expected to become an evaluation method of platelet function in vivo.

摘要

评估各种血栓性疾病中血小板活化程度对于检测血栓形成倾向和监测抗血小板治疗很重要。各种方法（血小板因子4：PF4、β-血小板球蛋白：β-TG、CD62P、PAC-1等）被用作血小板活化的标志物。血小板衍生微粒（PDMP）是血小板在活化过程中释放的亚微观囊泡，用作血小板活化的标志物。PDMP通常通过流式细胞术测定。最近报道了一种酶联免疫吸附测定（ELISA）方法，即用CD42a和CD42b抗体检测PDMP。用于ELISA的PDMP样本可以保存，标准化也很容易。它有望成为体内血小板功能的评估方法。