印度喀拉拉邦绵羊和山羊中蓝舌病毒抗体的血清流行率。

Seroprevalence of bluetongue virus antibodies in sheep and goats in Kerala State, India.

作者信息

Ravishankar C, Krishnan Nair G, Mini M, Jayaprakasan V

机构信息

Department of Microbiology, College of Veterinary and Animal Sciences, Mannuthy 680 651, Kerala, India.

出版信息

Rev Sci Tech. 2005 Dec;24(3):953-8.

PMID:16642765

Abstract

The results presented here record the first confirmation of bluetongue virus (BTV) antibody in sheep and goats in Kerala State. A total of 1,010 sera collected from the 14 districts within the state were screened for the presence of group-specific BTV antibodies by dot enzyme-linked immunosorbent assay (dot ELISA). Positive samples were obtained from 12 of the 14 districts. The overall BTV antibody prevalence was 5.1 +/- 1.9% (at 95% confidence level) although the prevalence levels were consistently higher in organised farms than in the field population. Comparative tests carried out using the dot ELISA and competitive ELISA (C ELISA) showed a good agreement for all the positive sera. The dot ELISA was simple to perform, economic and rapid, and is therefore ideally suited for routine screening for BTV antibody at the farm level.

摘要

此处呈现的结果首次证实了喀拉拉邦绵羊和山羊体内存在蓝舌病病毒（BTV）抗体。通过斑点酶联免疫吸附测定（斑点ELISA）对该邦14个区采集的共计1010份血清进行了检测，以筛查是否存在BTV群特异性抗体。在14个区中的12个区获得了阳性样本。尽管在集约化养殖场中BTV抗体流行率始终高于散养群体，但总体BTV抗体流行率为5.1±1.9%（95%置信水平）。使用斑点ELISA和竞争ELISA（C ELISA）进行的对比试验表明，所有阳性血清结果吻合度良好。斑点ELISA操作简单、经济且快速，因此非常适合在养殖场层面进行BTV抗体的常规筛查。