Karaman Ihsan M, Kaya Cevdet, Ozturk Metin, Pirincci Necip, Yimazgumrukcu Gulistan, Tuken Murat
Department of Urology, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey.
BJU Int. 2006 May;97(5):1116-8. doi: 10.1111/j.1464-410X.2006.06139.x.
OBJECTIVE To investigate the effect of human chorionic gonadotrophin (hCG) on rat testicular tissue, and its reversibility and dose dependence. MATERIALS AND METHODS Male Wistar rats were assigned to groups (10 rats/group) receiving 10, 30 or 50 IU/kg hCG subcutaneously once daily for 15 days; 10 controls received subcutaneous isotonic saline. At 1 and 3 months later, five rats in each group were killed and their testes removed. The testes were examined histologically to measure seminiferous tubular diameter and germinal membrane thickness. RESULTS At 1 month after hCG administration, the mean germinal membrane thickness in the testicular tissues of the hCG-treated rats was significantly less than in control rats, and was also significantly different between all of the hCG-treated groups (P < 0.05). However, at 3 months after hCG administration, all histological variables were similar to those in control rats (P > 0.05), and the mean germinal membrane thickness at 3 months after hCG administration was larger than that at 1 month (P < 0.05). There were no significant differences in the mean seminiferous tubular diameter between hCG-treated rats and control rats. CONCLUSION hCG impairs seminiferous tubule histology in the 'normal' descended testes of rats. This effect was dose-dependent, and the changes were reversed at 3 months after treatment. Thus, although hCG therapy might affect the seminiferous tubules of contralateral descended testes in cryptorchid boys, these effects might be reversible.
目的 研究人绒毛膜促性腺激素（hCG）对大鼠睾丸组织的影响及其可逆性和剂量依赖性。材料与方法 将雄性Wistar大鼠分为几组（每组10只大鼠），每天皮下注射10、30或50 IU/kg hCG，共15天；10只对照大鼠皮下注射等渗盐水。在1个月和3个月后，每组处死5只大鼠并摘除睾丸。对睾丸进行组织学检查，以测量曲细精管直径和生精膜厚度。结果 在给予hCG 1个月后，hCG处理组大鼠睾丸组织中生精膜的平均厚度显著低于对照组大鼠，且所有hCG处理组之间也存在显著差异（P < 0.05）。然而，在给予hCG 3个月后，所有组织学变量均与对照组大鼠相似（P > 0.05），且给予hCG 3个月后的生精膜平均厚度大于1个月时（P < 0.05）。hCG处理组大鼠与对照组大鼠的曲细精管平均直径无显著差异。结论 hCG会损害大鼠“正常”下降睾丸的曲细精管组织学。这种作用具有剂量依赖性，且在治疗3个月后变化逆转。因此，尽管hCG治疗可能会影响隐睾男孩对侧下降睾丸的曲细精管，但这些影响可能是可逆的。
